Anti-Claudin 5/CLDN5 Antibody

Product Name

Anti-Claudin 5/CLDN5 Antibody

View all Claudin-5 Antibodies

SKU/Catalog Number

RP1043

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Claudin 5/CLDN5 Antibody catalog # RP1043. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Claudin 5/CLDN5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1043)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5, different from the related mouse sequence by three amino acids and from the related rat sequence by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1043 is reactive to CLDN5 in Human, Rat

Applications

RP1043 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.147kDa

Background of Claudin-5

Claudin-5 (CLDN5) also known as BEC1, is a protein that in humans is encoded by the CLDN5 gene. By use of yeast artificial chromosomes, Claudin-5 is localized between polymorphic markers D22S944 and D22S941 on chromosome 22q11, both of which are deleted in more than 80% of VCFS patients. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. What's more, it plays a major role in tight junction-specific obliteration of the intercellular space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human

Validation Images & Assay Conditions

Click image to see more details

Anti-Claudin 5 Picoband antibody, RP1043, Western blotting
All lanes: Anti Claudin-5 (RP1043) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD

Gene/Protein Information For CLDN5 (Source: Uniprot.org, NCBI)

Alternative Names

AWAL; AWALTransmembrane protein deleted in VCFS; BEC1; claudin 5; Claudin5; Claudin-5; CLDN5; CPETRL1; TMDVCF; TMVCF1; TMVCFTMDVCF; transmembrane protein deleted in velocardiofacial syndrome CLDN5 AWAL, BEC1, CPETRL1, TMDVCF, TMVCF claudin 5 claudin-5|transmembrane protein deleted in VCFS|transmembrane protein deleted in velocardiofacial syndrome

For more info on CLDN5, check out the CLDN5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CLDN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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