Anti-ClC-5/CLCN5 Antibody Picoband™

Product Name

Anti-ClC-5/CLCN5 Antibody Picoband™

View all CLCN5 Antibodies

SKU/Catalog Number

A02286-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ClC-5/CLCN5 Antibody Picoband™ catalog # A02286-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ClC-5/CLCN5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02286-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CLCN5 recombinant protein (Position: R100-S756).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02286-2 is reactive to CLCN5 in Human, Mouse, Rat

Applications

A02286-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

100 kDa

Calculated molecular weight

83.147kDa

Background of CLCN5

The CLCN5 gene encodes the chloride channel Cl-/H+ exchanger ClC-5. This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ClC-5/CLCN5 using anti-ClC-5/CLCN5 antibody (A02286-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ClC-5/CLCN5 antigen affinity purified polyclonal antibody (Catalog # A02286-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ClC-5/CLCN5 at approximately 100 kDa. The expected band size for ClC-5/CLCN5 is at 91 kDa.

Gene/Protein Information For CLCN5 (Source: Uniprot.org, NCBI)

Alternative Names

chloride channel 5; Chloride channel protein 5; Chloride transporter ClC-5; CLC5; clC-5; CLCK2NPHL2; DENTSNPHL1; H(+)/Cl(-) exchange transporter 5; hCIC-K2; hClC-K2; nephrolithiasis 1 (X-linked); nephrolithiasis 2, X-linked; XLRH; XRN CLCN5 CLC5, CLCK2, ClC-5, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2 chloride voltage-gated channel 5 H(+)/Cl(-) exchange transporter 5|chloride channel, voltage-sensitive 5|chloride transporter ClC-5|voltage-gated chloride ion channel CLCN5

For more info on CLCN5, check out the CLCN5 Infographic

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In this infographic, you will see the following information for CLCN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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