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Product Info Summary
| SKU: | A02720-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CLC-7 CLCN7 Antibody
SKU/Catalog Number
A02720-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CLC-7 CLCN7 Antibody catalog # A02720-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLC-7 CLCN7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02720-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CLCN7. AA range:10-59
Reactive Species
A02720-1 is reactive to CLCN7 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
88.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02720-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of the lysates from COLO205 cells using CLCN7 antibody.
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Western Blot analysis of A549 cells using CLC-7 Polyclonal Antibody diluted at 1:500
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Western blot analysis of CLCN7 Antibody. The lane on the right is blocked with the CLCN7 peptide.
Specific Publications For Anti-CLC-7 CLCN7 Antibody (A02720-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CLC-7 CLCN7 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CLC-7 antibody A02720-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-18
Question
Would anti-CLC-7 antibody A02720-1 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of placenta, CLCN7 is highly expressed in placenta. So, it is likely that anti-CLC-7 antibody A02720-1 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-09-25
Question
We are currently using anti-CLC-7 antibody A02720-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The anti-CLC-7 antibody (A02720-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-01
Question
I see that the anti-CLC-7 antibody A02720-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-23
Question
Would A02720-1 anti-CLC-7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
You can see on the product datasheet, A02720-1 anti-CLC-7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-13
Question
See attached the WB image, lot number and protocol we used for placenta using anti-CLC-7 antibody A02720-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-17
Question
Can you help my question with product A02720-1, anti-CLC-7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Krishna
Verified customer
Asked: 2017-05-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02720-1 anti-CLC-7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-12
