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Product Info Summary
| SKU: | M00334-11 |
|---|---|
| Size: | 20μL, 40μL, 100μL, 200μL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-Cleaved Caspase-3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00334-11
Size
20μL, 40μL, 100μL, 200μL
Description
This Recombinant Rabbit Monoclonal Antibody detects endogenous levels of Cleaved Caspase-3 (Asp175) protein. Validated for research with WB,IHC,IF,IP,ELISA applications. Anti Cleaved Caspase-3 (Asp175) is reactive for Human, Mouse, Rat samples
Storage & Handling
Store at -20°C/1 year
Cite This Product
Anti-Cleaved Caspase-3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-11)
Host
Rabbit
Contents
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% stabilizing protein
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG, Kappa
Reactive Species
M00334-11 is reactive to CASP3 in Human, Mouse, Rat
Calculated molecular weight
31.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00334-11 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200-1:1000
WB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
Validation Images & Assay Conditions
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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-3 (Asp175) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: NIH-3T3
Lane 2: NIH-3T3 tearted with Apopida
Predicted band size: 17,19kDa
Observed band size: 17,19kDa
Specific Publications For Anti-Cleaved Caspase-3 Rabbit Monoclonal Antibody (M00334-11)
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