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Product Info Summary
| SKU: | A00624 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cleaved-COL1A2 (G1102) Antibody
SKU/Catalog Number
A00624
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-COL1A2 (G1102) Antibody catalog # A00624. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-COL1A2 (G1102) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00624)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Collagen I alpha2. AA range:1053-1102
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00624 is reactive to COL1A2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
129.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00624 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of VEC cells using Cleaved-COL1A2 (G1102) Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Collagen I alpha2 (Cleaved-Gly1102) Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-Cleaved-COL1A2 (G1102) Antibody (A00624)
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Customer Q&As
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3 Customer Q&As for Anti-Cleaved-COL1A2 (G1102) Antibody
Question
Will A00624 anti-Cleaved-COL1A2 (G1102) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Krishna
Verified customer
Asked: 2020-03-05
Answer
You can see on the product datasheet, A00624 anti-Cleaved-COL1A2 (G1102) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-05
Question
Is there a BSA free version of anti-Cleaved-COL1A2 (G1102) antibody A00624 available?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Cleaved-COL1A2 (G1102) antibody A00624 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-12
Question
Is a blocking peptide available for product anti-Cleaved-COL1A2 (G1102) antibody (A00624)?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We do provide the blocking peptide for product anti-Cleaved-COL1A2 (G1102) antibody (A00624). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-16
