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Product Info Summary
| SKU: | A07196 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Cleaved-ITI-H2 (D702) Antibody
SKU/Catalog Number
A07196
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cleaved-ITI-H2 (D702) Antibody catalog # A07196. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cleaved-ITI-H2 (D702) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07196)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ITIH2. AA range:653-702
Reactive Species
A07196 is reactive to ITIH2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
106.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07196 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Cleaved-ITI-H2 (D702) Polyclonal antibody.
Specific Publications For Anti-Cleaved-ITI-H2 (D702) Antibody (A07196)
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Customer Q&As
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6 Customer Q&As for Anti-Cleaved-ITI-H2 (D702) Antibody
Question
Does anti-Cleaved-ITI-H2 (D702) antibody A07196 work on bovine WB with serum?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Our lab technicians have not validated anti-Cleaved-ITI-H2 (D702) antibody A07196 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cleaved-ITI-H2 (D702) antibody A07196 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine serum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
We are currently using anti-Cleaved-ITI-H2 (D702) antibody A07196 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-Cleaved-ITI-H2 (D702) antibody (A07196) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
Our lab want to know about to test anti-Cleaved-ITI-H2 (D702) antibody A07196 on human plasma for research purposes, then I may be interested in using anti-Cleaved-ITI-H2 (D702) antibody A07196 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-06
Answer
The products we sell, including anti-Cleaved-ITI-H2 (D702) antibody A07196, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-06
Question
I have a question about product A07196, anti-Cleaved-ITI-H2 (D702) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Brown
Verified customer
Asked: 2018-04-17
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07196 anti-Cleaved-ITI-H2 (D702) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-17
Question
Will anti-Cleaved-ITI-H2 (D702) antibody A07196 work for WB with plasma?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
According to the expression profile of plasma, ITIH2 is highly expressed in plasma. So, it is likely that anti-Cleaved-ITI-H2 (D702) antibody A07196 will work for WB with plasma.
Boster Scientific Support
Answered: 2018-03-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Cleaved-ITI-H2 (D702) antibody A07196. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-13
