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Product Info Summary
| SKU: | A03271 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CLK1 Antibody
SKU/Catalog Number
A03271
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CLK1 Antibody catalog # A03271. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03271)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CLK1. AA range:101-150
Reactive Species
A03271 is reactive to CLK1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03271 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from 293 cells, using CLK1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HUVEC cells, using CLK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CLK1 Antibody (A03271)
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Customer Reviews
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5 Customer Q&As for Anti-CLK1 Antibody
Question
I was wanting to use your anti-CLK1 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It shows on the product datasheet, A03271 anti-CLK1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-26
Question
Is a blocking peptide available for product anti-CLK1 antibody (A03271)?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
We do provide the blocking peptide for product anti-CLK1 antibody (A03271). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-19
Question
Will anti-CLK1 antibody A03271 work on bovine WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-04-09
Answer
Our lab technicians have not tested anti-CLK1 antibody A03271 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CLK1 antibody A03271 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-09
Question
I would like to test anti-CLK1 antibody A03271 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-CLK1 antibody A03271 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Baker
Verified customer
Asked: 2017-05-05
Answer
The products we sell, including anti-CLK1 antibody A03271, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-05
Question
We are currently using anti-CLK1 antibody A03271 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
G. Bhatt
Verified customer
Asked: 2013-11-19
Answer
The anti-CLK1 antibody (A03271) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-19
