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Product Info Summary
| SKU: | A04566 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-COH1 VPS13B Antibody
SKU/Catalog Number
A04566
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-COH1 VPS13B Antibody (Catalog # A04566). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
COH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-COH1 VPS13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04566)
Host
Rabbit
Contents
COH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1. The immunogen is located within amino acids 90 - 140 of COH1.
Cross-reactivity
At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms.
Reactive Species
A04566 is reactive to VPS13B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
448.7 kDa
Background of VPS13B
COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04566 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of COH1 in human brain tissue with COH1 antibody at 20 μg/mL.
Specific Publications For Anti-COH1 VPS13B Antibody (A04566)
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