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- Table of Contents
COL1A1 antibody (M00624) performs well in IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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IHC analysis of Collagen I using anti-Collagen I antibody (M00624).
Collagen I was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Collagen I Antibody (M00624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Collagen I was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Collagen I Antibody (M00624) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody
COL1A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IHC, WB |
Tested Applications
COL1A1 antibody was positively detected by WB in:
Human stomach tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
COL1A1 antibody was positively detected by IHC in:
human breast cancer tissue, human colorectal adenocarcinoma tissue, human lung squamous cell carcinoma tissue, human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody
View all COL1A1 Antibodies
View all COL1A1 Antibodies
Form
Liquid
Uniprot
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Collagen I
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
139 kDa
Cite This Product
Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00624)
Protocols
Western Blot protocol for COL1A1 antibody
Immunohistochemistry (IHC) protocol for COL1A1 antibody
Publications
Application:
Species:
SnRNA-seq of skeletal muscles reveals the cellular and molecular landscapes for meat quality and locomotor of Mongolian cattle
Published: 2026-08-19
Journal: BMC GENOMICS
Application: IF
Impact Factor: 3.90
Species: Mouse
FAP+ gastric cancer mesenchymal stromal cells via paracrining INHBA and remodeling ECM promote tumor progression
Published: 2024-11-29
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Human
PMID: 39615112
Investigating the role of various extracellular matrix in androgenetic alopecia: Insights from immunostaining and quantitative analysis – A pilot study
Published: 2024-10-10
Journal: Dermatologica Sinica
Application: IHC
Impact Factor: 2.30
Species: Human
Arginine-loaded globular BSAMA/fibrous GelMA biohybrid cryogels with multifunctional features and enhanced healing for soft gingival tissue regeneration
Published: 2024-08-22
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Application: WB
Impact Factor: 7.70
Species: Human,Rat
PMID: 39179087
Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway
Published: 2024-06-25
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: IF,WB
Impact Factor: 4.80
Species: Mouse
PMID: 38924867
A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signaling
Published: 2022-01-21
Journal: Nature Communications
Application: WB
Impact Factor: 17.69
Species: Mouse
PMID: 35064106
MicroRNA-21 via Dysregulation of WW Domain-Containing Protein 1 Regulate Atrial Fibrosis in Atrial Fibrillation
Published: 2017-03-12
Journal: Heart Lung and Circulation
Application: WB
Impact Factor: 2.03
Species: Human
PMID: 28495464
miR-200a controls hepatic stellate cell activation and fibrosis via SIRT1/Notch1 signal pathway
Published: 2016-12-26
Journal: INFLAMMATION RESEARCH
Application: WB
Impact Factor: 2.56
Species: Rat
PMID: 28025657
A novel synthesized sulfonamido-based gallic acid – LDQN-C: Effects on chondrocytes growth and phenotype maintenance
Published: 2014-09-30
Journal: BIOORGANIC CHEMISTRY
Application: IHC
Impact Factor: 2.14
Species: Rat
PMID: 25305720
Effect of JEZTC, a synthetic compound, on proliferation and phenotype maintenance of rabbit articular chondrocytes in vitro
Published: 2014-08-15
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: ICC
Impact Factor: 1.00
Species: Rabbit
PMID: 25124868
Review
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Product Promise
We promise Anti-Collagen I COL1A1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00624
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026