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- Table of Contents
COL3A1 antibody (M00788-1) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Western blot analysis of Collagen III expression in (1) A431 cell lysate; (2) MCF7 cell lysate.
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Anti-Collagen III Rabbit Monoclonal Antibody
COL3A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Collagen III Rabbit Monoclonal Antibody
View all COL3A1 Antibodies
View all COL3A1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
26C74
Isotype
IgG
Immunogen
A synthesized peptide derived from Collagen III
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
150 kDa-200 kDa
Calculated Molecular Weight
138.6 kDa
Cite This Product
Anti-Collagen III Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00788-1)
Publications
Application:
Species:
Enzymatic self-assembly nanofibers anchoring mesenchymal stem cells induce cell spheroids and amplify paracrine function for myocardial infarction therapy
Published: 2022-02-12
Journal: CHEMICAL ENGINEERING JOURNAL
Application: IF
Impact Factor: 16.74
Species: Mouse
Glycemic control is associated with atrial structural remodeling in patients with type 2 diabetes
Published: 2019-12-02
Journal: BMC Cardiovascular Disorders
Application: WB
Impact Factor: 1.95
Species: Human
PMID: 31791258
Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings
Published: 2019-09-23
Journal: Stem Cell Research & Therapy
Application: ICC
Impact Factor: 4.63
Species: Human
PMID: 31547864
MicroRNA-21 via Dysregulation of WW Domain-Containing Protein 1 Regulate Atrial Fibrosis in Atrial Fibrillation
Published: 2017-03-12
Journal: Heart Lung and Circulation
Application: WB
Impact Factor: 2.03
Species: Human
PMID: 28495464
Effect of Rougan Huaqian granules combined with human mesenchymal stem cell transplantation on liver fibrosis in cirrhosis rats
Published: 2014-07-23
Journal: Asian Pacific Journal of Tropical Medicine
Application: WB
Impact Factor: 0.93
Species: Rat
PMID: 25063290
β-catenin is overexpressed in hepatic fibrosis and blockage of Wnt/β-catenin signaling inhibits hepatic stellate cell activation
Published: 2014-04-01
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 1.48
Species: Rat
PMID: 24691643
Review
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2 Reviews For Anti-Collagen III Rabbit Monoclonal Antibody
This Western blot experiment using COL3A1 antibody (M00788-1) on mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.
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| SKU | M00788-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse cardiac tissue |
| Sample Processing Description | Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Collagen III Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h at RT |
| Detection | Substrate: ECL substrate, Image system: ChemiDoc MP |
| Results Summary | This experiment aimed to investigate changes in COL3A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses. |
Huanping Qian, Ningxia Medical University
Verified customer
Submitted 2026-03-24
Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.
| SKU | M00788-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse brain tissue |
| Sample Processing Description | Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer |
| Primary Antibody | Collagen III Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses. |
Huanping Qian, Ningxia Medical University
Verified customer
Submitted 2026-03-05
Product Promise
We promise Anti-Collagen III Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00788-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026