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Product Info Summary
| SKU: | M00788-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-Collagen III Rabbit Monoclonal Antibody
SKU/Catalog Number
M00788-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Collagen III Rabbit Monoclonal Antibody catalog # M00788-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen III Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00788-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
26C74
Isotype
IgG
Immunogen
A synthesized peptide derived from Collagen III
Reactive Species
M00788-1 is reactive to COL3A1 in Human, Mouse, Rat
Observed Molecular Weight
150 kDa-200 kDa
Calculated molecular weight
138.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00788-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Collagen III expression in (1) A431 cell lysate; (2) MCF7 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Collagen III using anti-Collagen III antibody (M00788-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: cardiac tissue lysates from 3-month-old mice,
Lane 2: cardiac tissue lysates from 6-month-old mice,
Lane 3: cardiac tissue lysates from 12-month-old mice,
Lane 4: cardiac tissue lysates from 18-month-old mice.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III antigen affinity purified polyclonal antibody (Catalog # M00788-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Collagen III at approximately 139 kDa. The expected band size for Collagen III is at 138 kDa.
Specific Publications For Anti-Collagen III Rabbit Monoclonal Antibody (M00788-1)
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Customer Reviews
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2 Reviews For Anti-Collagen III Rabbit Monoclonal Antibody
This Western blot experiment using COL3A1 antibody (M00788-1) on mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice shows that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses.
Excellent
| SKU | M00788-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse cardiac tissue |
| Sample Processing Description | Total protein was extracted from mouse cardiac tissues at 3, 6, 12, and 18 months of age. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Collagen III Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h at RT |
| Detection | Substrate: ECL substrate, Image system: ChemiDoc MP |
| Results Summary | This experiment aimed to investigate changes in COL3A1 expression in mouse cardiac tissues from 3-, 6-, 12-, and 18-month-old mice (representing young, middle-aged, middle-old, and old stages), and the results show that COL3A1 levels are generally similar across ages, with a slight but not significant increase as age progresses. |
Huanping Qian, Ningxia Medical University
Verified customer
Submitted 2026-03-24
Western blot analysis using Anti-COL3A1 Antibody (M00788-1) showed clear and specific bands in mouse myocardium samples from 3-, 6-, 12-, and 18-month-old mice, with overall comparable COL3A1 expression and a slight, non-significant increase with age.
Excellent
| SKU | M00788-1 |
|---|---|
| Application | Western Blot |
| Sample | mouse brain tissue |
| Sample Processing Description | Myocardial tissues were collected from mice at 3, 6, 12, and 18 months of age, and total protein was extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, blocking buffer |
| Primary Antibody | Collagen III Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system:ChemiDoc MP |
| Results Summary | This experiment aimed to study the changes in COL3A1 protein in mouse myocardium at 3, 6, 12, and 18 months of age (representing young, adult, middle-aged, and old mice). The results show that COL3A1 levels are largely similar across ages, with a slight, non-significant increase as age progresses. |
Huanping Qian, Ningxia Medical University
Verified customer
Submitted 2026-03-05
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