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COL4A1 antibody (PB9099) performs well in IF, IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 24 Publications

pb9099 2 IHC anti collagen iv picoband antibody

IHC analysis of Collagen using anti-Collagen antibody (PB9099).
Collagen was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Anti-Collagen IV/COL4A1 Antibody Picoband®

COL4A1 antibody

24 Publications

Reactive Species	Host	Application
Human	Rabbit	IF, IHC, WB

Tested Applications

COL4A1 antibody was positively detected by WB in:

human placenta tissue,

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

COL4A1 antibody was positively detected by IHC in:

human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

COL4A1 antibody was positively detected by IF in:

human lung cancer tissue, human colon cancer tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Mouse

Predicted

Rat

Predicted

Product Information

Product Name

Anti-Collagen IV/COL4A1 Antibody Picoband®
View all COL4A1 Antibodies

Form

Lyophilized

Uniprot

P02462

Gene ID

1282

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

250 kDa

Calculated Molecular Weight

160.6 kDa

Background of COL4A1

COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Cite This Product

Anti-Collagen IV/COL4A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9099)

Protocols

Western Blot protocol for COL4A1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for COL4A1 antibody

Review Protocol

Immunofluorescence (IF) protocol for COL4A1 antibody

Review Protocol

Publications

Therapeutic potential of recombinant human collagen XVII in blue light-induced skin photoaging: preserving epidermal-dermal structural integrity and functional homeostasis

Published: 2026-04-28

Journal: Frontiers in Bioengineering and Biotechnology

Application: WB

Impact Factor: 4.80

Species: Human

PMID: 42131503

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Anti-Collagen IV/COL4A1 Antibody Picoband®

COL4A1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Therapeutic potential of recombinant human collagen XVII in blue light-induced skin photoaging: preserving epidermal-dermal structural integrity and functional homeostasis

XPD Regulates MIAT/miR-29a-3p/COL4A1 Axis to Impede Hepatocellular Carcinoma Development

The role of leukotriene B in cow metritis

Ibrolipim attenuates early-stage nephropathy in diet-induced diabetic minipigs: Focus on oxidative stress and fibrogenesis

Revealing the pathogenic changes of PAH based on multiomics characteristics

Biocompatibility of injectable hydrogel from decellularized human adipose tissue in vitro and in vivo

Cardioprotection of Sheng Mai Yin a classic formula on adriamycin induced myocardial injury in Wistar rats

Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis

Sesamin Ameliorates High-Fat Diet–Induced Dyslipidemia and Kidney Injury by Reducing Oxidative Stress

Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus

Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation

Effects of uPA on mesangial matrix changes in the kidney of diabetic rats

Resveratrol Attenuates Diabetic Nephropathy via Modulating Angiogenesis

Electrosprayed Microparticles with Loaded pDNA-Calcium Phosphate Nanoparticles to Promote the Regeneration of Mature Blood Vessels

Transcriptome Sequencing Reveals Differences between Primary and Secondary Hair Follicle-derived Dermal Papilla Cells of the Cashmere Goat (Capra hircus)

Renoprotective effects of berberine and its possible molecular mechanisms in combination of high-fat diet and low-dose streptozotocin-induced diabetic rats

Overexpressed C-type natriuretic peptide serves as an early compensatory response to counteract extracellular matrix remodeling in unilateral ureteral obstruction rats

The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis

Advanced Glycation End-Products Induce Connective Tissue Growth Factor-Mediated Renal Fibrosis Predominantly through Transforming Growth Factor β-Independent Pathway

Effects of aminoguanidine and vitamin C on collagen type IV in diabetic nephropathy rats

Biocompatibility of pure titanium modified by human endothelial cell-derived extracellular matrix

Severe Insulin Resistance and Moderate Glomerulosclerosis in a Minipig Model Induced by High-Fat/ High-Sucrose/ High-Cholesterol Diet

Rosiglitazone Prevents Advanced Glycation End Products–Induced Renal Toxicity Likely through Suppression of Plasminogen Activator Inhibitor-1

Protective effects of blocking renin-angiotensin system on the progression of renal injury in glomerulosclerosis.

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