SKU MA1030
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number COL-94
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC

Overview

Product Name Anti-Collagen, Type IV Antibody (Monoclonal, COL-94)
SKU/Catalog Number MA1030
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Collagen, Type IV, collagen, type IV, alpha-1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen,type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6 (COL4A1; COL4A3; COL4A4; COL4A5; COL4A6) detection. Tested with IHC-P, IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-Collagen, Type IV Antibody (Monoclonal, COL-94) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1030)
Host Mouse
Immunogen Human collagen type IV.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1030 1 IHC anti collagen type iv antibody monoclonal col 94.jpg

Anti-Collagen, Type IV antibody (monoclonal), MA1030, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P02462
Gene Name COL4A1
Protein Name Collagen alpha-1(IV) chain
Tissue Specificity Highly expressed in placenta. .
Alternative Names Collagen alpha-1(IV) chain;Arresten;COL4A1;
Subcellular Localization Secreted, extracellular space, extracellular matrix, basement membrane.
Molecular Weight 160615 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Background Collagen IV is comprised of a third chain(alpha-3) together with the 2 classical ones, alpha-1 and alpha-2. COL4A3 gene is localized to 2q36-q37 by analysis of somatic cell hybrids and by in situ hybridization. The alpha-3 chain of type IV collagen induces autoimmune Goodpasture syndrome. Mutations in the COL4A4 and COL4A3 genes cause familial benign hematuria.

Order Product (MA1030)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for MA1030

Publications

Zhang Z, Wu F, Zheng F, Li H. Nephron Exp Nephrol. 2010;116(1):E11-21. Doi: 10.1159/000314669. Epub 2010 May 21. Adenovirus-Mediated Decorin Gene Transfection Has Therapeutic Effects In A Streptozocin-Induced Diabetic Rat Model.
Wen D, Huang X, Zhang M, Zhang L, Chen J, Gu Y, Hao Cm. Plos One. 2013 Dec 3;8(12):E82336. Doi: 10.1371/Journal.Pone.0082336. Ecollection 2013. Resveratrol Attenuates Diabetic Nephropathy Via Modulating Angiogenesis.
Xu L, Zheng N, He Q, Li R, Zhang K, Liang T. Phytomedicine. 2013 Oct 15;20(13):1172-9. Doi: 10.1016/J.Phymed.2013.06.008. Epub 2013 Jul 13. Puerarin, Isolated From Pueraria Lobata (Willd.), Protects Against Hepatotoxicity Via Specific Inhibition O...
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.