Anti-COLQ Antibody

COLQ antibody

Boster Bio Anti-COLQ Antibody (Catalog# A03301). Tested in IHC-P, IF (paraffin), WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03301
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC-P, WB, IF (paraffin)

Product Name

Anti-COLQ Antibody

View all COLQ Antibodies

SKU/Catalog Number

A03301

Size

100ul

Form

Liquid

Description

Boster Bio Anti-COLQ Antibody (Catalog# A03301). Tested in IHC-P, IF (paraffin), WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COLQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03301)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

7A7

Isotype

Rabbit IgG

Immunogen

A peptide derived from human COLQ. Immunogen sequence location: 189 - 238

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03301 is reactive to COLQ in Human, Mouse, Rat

Applications

A03301 is guaranteed for IHC-P, WB, IF (paraffin) Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

47.766kDa

Background of COLQ

Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC-P, 1:50 - 1:200
Western Blot, 1:500 - 1:2000

Validation Images & Assay Conditions

Gene/Protein Information For COLQ (Source: Uniprot.org, NCBI)

Gene Name

COLQ

Full Name

Acetylcholinesterase collagenic tail peptide

Weight

47.766kDa

Superfamily

COLQ family

Alternative Names

acetylcholinesterase collagenic tail peptide; AChE Q subunit; collagenic tail of endplate acetylcholinesterase; collagen-like tail subunit (single strand of homotrimer) of asymmetricacetylcholinesterase; EADAcetylcholinesterase-associated collagen; FLJ55041; single strand of homotrimeric collagen-like tail subunit of asymmetricacetylcholinesterase COLQ CMS5, EAD collagen like tail subunit of asymmetric acetylcholinesterase acetylcholinesterase collagenic tail peptide|AChE Q subunit|acetylcholinesterase-associated collagen|collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase|collagenic tail of endplate acetylcholinesterase|single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on COLQ, check out the COLQ Infographic

COLQ infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for COLQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-COLQ Antibody

Question

I have a question about product A03301, anti-COLQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03301 anti-COLQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-08

Question

Would A03301 anti-COLQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Anderson

Verified customer

Asked: 2019-04-12

Answer

As indicated on the product datasheet, A03301 anti-COLQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-12

Question

We are currently using anti-COLQ antibody A03301 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-07

Answer

The anti-COLQ antibody (A03301) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-07

Question

Is a blocking peptide available for product anti-COLQ antibody (A03301)?

K. Krishna

Verified customer

Asked: 2015-02-13

Answer

We do provide the blocking peptide for product anti-COLQ antibody (A03301). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-02-13

Question

I was wanting to use your anti-COLQ antibody for WB for rat skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skeletal muscle identification?

Z. Thomas

Verified customer

Asked: 2013-04-29

Answer

You can see on the product datasheet, A03301 anti-COLQ antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skeletal muscle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-29

Order DetailsPrice
A03301

100 μL

$335

Free Secondary Antibody

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A03301
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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