Overview
| Product Name |
Anti-Complement C3 Monoclonal Antibody
See more C3 products |
| Catalog# |
M00168-2 |
| Pack Size |
100ug |
| Storage & Handling |
At -20°C for one year. Avoid repeated freezing and thawing. |
| Description |
Rabbit IgG Monoclonal antibody for Complement C3 detection. Tested positive for WB, IP in Human. |
| Cite This Product |
Anti-Complement C3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00168-2)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
IP, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human C3 / C3a |
Gene/Protein Basic Information For C3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P01024 |
|---|
| NCBI Gene Id | 718 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | C3 |
|---|
| Protein Name | Complement C3 |
|---|
| Alternative Names | C3|AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; complement C3; complement component 3; complement component C3; CPAMD1acylation-stimulating protein cleavage product |
|---|
| Molecular Weight | 187148 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on C3, check out the C3 Infographic
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In this infographic you will see the following information for C3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the C3 infographic
Our Boster Quality Guarantee for Anti-Complement C3 Monoclonal Antibody covers its use in the following applications.
WB 1:500~1:2000
IP 1:50
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of C3 expression in Human plasma lysate using anti-C3 antibody (M00168-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # M00168-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for C3.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Total number of citations: 0
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Questions and answers from customers related to M00168-2 Anti-Complement C3 Monoclonal Antibody
4 Related Questions
Question
Is this M00168-2 anti-Complement C3 Monoclonal antibody reactive to the isotypes of C3?
F. Miller
Asked: 2019-04-17
Answer
The immunogen of M00168-2 anti-Complement C3 Monoclonal antibody is A synthesized peptide derived from human C3 / C3a C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-17
Question
We are currently using anti-Complement C3 Monoclonal antibody M00168-2 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Asked: 2018-05-21
Answer
The anti-Complement C3 Monoclonal antibody (M00168-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-21
Question
I was wanting to use your anti-Complement C3 Monoclonal antibody for WB for human parietal pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human parietal pleura identification?
D. Baker
Asked: 2017-09-14
Answer
As indicated on the product datasheet, M00168-2 anti-Complement C3 Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parietal pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-14
Question
I have a question about product M00168-2, anti-Complement C3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Baker
Asked: 2014-08-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00168-2 anti-Complement C3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-08-27
