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Product Info Summary
| SKU: | M00168-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Complement C3 Monoclonal Antibody
SKU/Catalog Number
M00168-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Complement C3 Monoclonal Antibody catalog # M00168-2. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Complement C3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00168-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human C3 / C3a
Reactive Species
M00168-2 is reactive to C3 in Human
Observed Molecular Weight
187 kDa
Calculated molecular weight
187.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00168-2 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:20
Validation Images & Assay Conditions
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Western blot analysis of C3 / C3a expression in Human plasma lysate.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Specific Publications For Anti-Complement C3 Monoclonal Antibody (M00168-2)
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4 Customer Q&As for Anti-Complement C3 Monoclonal Antibody
Question
Is this M00168-2 anti-Complement C3 Monoclonal antibody reactive to the isotypes of C3?
F. Miller
Verified customer
Asked: 2019-04-17
Answer
The immunogen of M00168-2 anti-Complement C3 Monoclonal antibody is A synthesized peptide derived from human C3 / C3a C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-04-17
Question
We are currently using anti-Complement C3 Monoclonal antibody M00168-2 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
The anti-Complement C3 Monoclonal antibody (M00168-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-21
Question
I was wanting to use your anti-Complement C3 Monoclonal antibody for WB for human parietal pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human parietal pleura identification?
D. Baker
Verified customer
Asked: 2017-09-14
Answer
As indicated on the product datasheet, M00168-2 anti-Complement C3 Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parietal pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-14
Question
I have a question about product M00168-2, anti-Complement C3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Baker
Verified customer
Asked: 2014-08-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00168-2 anti-Complement C3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-08-27
