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Product Info Summary
| SKU: | PA1368 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Connexin 40/GJA5 Antibody
View all Connexin 40/GJA5 Antibodies
SKU/Catalog Number
PA1368
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Connexin 40/GJA5 Antibody catalog # PA1368. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Connexin 40/GJA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1368)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 40/GJA5, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1368 is reactive to GJA5 in Human, Mouse, Rat
Applications
PA1368 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
50 kDa
Calculated molecular weight
40.38kDa
Background of Connexin 40/GJA5
Connexin proteins oligomerize to form intercellular channels, called gap junctions, through which ions and small molecules move between adjacent cells. Connexin 40 (Gap junction alpha-5 protein) is a protein that in humans is encoded by the GJA5 gene.This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat
Validation Images & Assay Conditions
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Anti-Connexin 40/GJA5 antibody, PA1368, Western blotting
Lane 1: Mouse Heart Tissue Lysate
Lane 2: Mouse Heart Tissue Lysate
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Anti-Connexin 40/GJA5 antibody, PA1368, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For GJA5 (Source: Uniprot.org, NCBI)
Gene Name
GJA5
Full Name
Gap junction alpha-5 protein
Weight
40.38kDa
Superfamily
connexin family
Alternative Names
alpha 5, 40kD (connexin 40); ATFB11; Connexin 40; connexin-40; CX40; gap junction alpha-5 protein; gap junction protein, alpha 5, 40kDa (connexin 40); gap junction protein, alpha 5, 40kDa; GJA5; MGC11185 GJA5 ATFB11, CX40 gap junction protein alpha 5 gap junction alpha-5 protein|connexin 40|gap junction protein alpha 5 40kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJA5, check out the GJA5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJA5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 40/GJA5 Antibody (PA1368)
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No publications found for PA1368
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Connexin 40/GJA5 Antibody
Question
We are currently using anti-Connexin 40/GJA5 antibody PA1368 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-Connexin 40/GJA5 antibody (PA1368) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
Will anti-Connexin 40/GJA5 antibody PA1368 work for IHC with placenta?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
According to the expression profile of placenta, GJA5 is highly expressed in placenta. So, it is likely that anti-Connexin 40/GJA5 antibody PA1368 will work for IHC with placenta.
Boster Scientific Support
Answered: 2019-09-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Connexin 40/GJA5 antibody PA1368. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-08
Question
Is there a BSA free version of anti-Connexin 40/GJA5 antibody PA1368 available?
S. Moore
Verified customer
Asked: 2013-05-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Connexin 40/GJA5 antibody PA1368 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-10
