Anti-COP1 RFWD2 Antibody

Boster Bio Anti-COP1 RFWD2 Antibody catalog # A04843. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04843
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-COP1 RFWD2 Antibody

See all COP1 products

SKU/Catalog Number

A04843

Size

100μl

Form

Liquid

Description

Boster Bio Anti-COP1 RFWD2 Antibody catalog # A04843. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COP1 RFWD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04843)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human COP1 around the non-phosphorylation site of S387.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04843 is reactive to RFWD2 in Human, Mouse

Applications

A04843 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For RFWD2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8NHY2

Gene ID

64326

Gene Name

RFWD2

Full Name

E3 ubiquitin-protein ligase COP1

Weight

80.474kDa

Superfamily

COP1 family

Alternative Names

E3 ubiquitin-protein ligase COP1 COP1 CFAP78, FAP78, RFWD2, RNF200 COP1 E3 ubiquitin ligase E3 ubiquitin-protein ligase COP1|E3 ubiquitin-protein ligase RFWD2|RING finger protein 200|RING-type E3 ubiquitin transferase RFWD2|constitutive photomorphogenesis protein 1 homolog|constitutive photomorphogenic protein 1|putative ubiquitin ligase COP1|ring finger and WD repeat domain 2, E3 ubiquitin protein ligase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RFWD2, check out the RFWD2 Infographic

RFWD2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RFWD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04843

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13 Customer Q&As for Anti-COP1 RFWD2 Antibody

Question

Would A04843 anti-COP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

You can see on the product datasheet, A04843 anti-COP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-16

Question

I have a question about product A04843, anti-COP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04843 anti-COP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-18

Question

I see that the anti-COP1 antibody A04843 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-26

Question

See below the WB image, lot number and protocol we used for testis {eco:0000313 using anti-COP1 antibody A04843. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-13

Question

Is a blocking peptide available for product anti-COP1 antibody (A04843)?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

We do provide the blocking peptide for product anti-COP1 antibody (A04843). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-13

Question

Would anti-COP1 antibody A04843 work on dog WB with testis {eco:0000313?

M. Li

Verified customer

Asked: 2019-08-19

Answer

Our lab technicians have not tested anti-COP1 antibody A04843 on dog. You can run a BLAST between dog and the immunogen sequence of anti-COP1 antibody A04843 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog testis {eco:0000313 in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-19

Question

We are interested in to test anti-COP1 antibody A04843 on mouse testis {eco:0000313 for research purposes, then I may be interested in using anti-COP1 antibody A04843 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

P. Williams

Verified customer

Asked: 2019-03-11

Answer

The products we sell, including anti-COP1 antibody A04843, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-11

Question

Is this A04843 anti-COP1 antibody reactive to the isotypes of RFWD2?

Verified Customer

Verified customer

Asked: 2018-07-11

Answer

The immunogen of A04843 anti-COP1 antibody is Synthesized peptide derived from human COP1 around the non-phosphorylation site of S387. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-07-11

Question

Would anti-COP1 antibody A04843 work for WB with testis {eco:0000313?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

According to the expression profile of testis {eco:0000313, RFWD2 is highly expressed in testis {eco:0000313. So, it is likely that anti-COP1 antibody A04843 will work for WB with testis {eco:0000313.

Boster Scientific Support

Answered: 2018-01-31

Question

I was wanting to use your anti-COP1 antibody for WB for mouse testis eco:0000313 on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been validated and is this antibody a good choice for mouse testis {eco:0000313 identification?

M. Mangal

Verified customer

Asked: 2015-04-06

Answer

You can see on the product datasheet, A04843 anti-COP1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis {eco:0000313 in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-04-06

Question

Is there a BSA free version of anti-COP1 antibody A04843 available?

S. Parker

Verified customer

Asked: 2015-02-10

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-COP1 antibody A04843 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-02-10

Question

We are currently using anti-COP1 antibody A04843 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?

J. Zhao

Verified customer

Asked: 2014-10-31

Answer

The anti-COP1 antibody (A04843) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis {eco:0000313 using anti-COP1 antibody A04843. Let me know if you need anything else.

J. Jones

Verified customer

Asked: 2013-11-11

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-11

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04843
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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