Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248

Product Name

Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248

View all Macroh2a1 Antibodies

SKU/Catalog Number

M04635-1

Size

100ug

Form

Liquid

Description

Boster Bio Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Catalog # M04635-1). Tested in WB, ICC, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Core Histone Macro-H2A.1 (H2AFY) Rabbit Monoclonal Antibody, Clone#RM248 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04635-1)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM248

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the C-terminus of human Histone macroH2A1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.

Reactive Species

M04635-1 is reactive to Macroh2a1 in Human, Vertebrates

Applications

M04635-1 is guaranteed for ELISA, ICC, WB, Luminex Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

39.735kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.5 ug/mL – 2 ug/mL
ICC: 1 μg/mL - 2 μg/mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.2 ug/mL – 1 ug/mL.

Validation Images & Assay Conditions

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Figure 1. Western Blotting result
Western Blot of acid extracts from K562 cells, using RM248 at 1 ug/mL

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Figure 2. ICC result
Immunocytochemistry of HeLa cells, using anti-Histone macroH2A1 Rabbit mAb RM248 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Gene/Protein Information For Macroh2a1 (Source: Uniprot.org, NCBI)

Alternative Names

Core histone macro-H2A.1 Macroh2a1|H2AF12, H2AF12M, H2af, H2afy, MACROH2, mH2a, mH2a1|macroH2A.1 histone|core histone macro-H2A.1|H2A histone family, member Y|H2A.y|H2A/y|MACROH2A1.2|histone macroH2A1

For more info on Macroh2a1, check out the Macroh2a1 Infographic

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In this infographic, you will see the following information for Macroh2a1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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