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Product Info Summary
| SKU: | M03833 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-COUP TF1 NR2F1 Monoclonal Antibody
View all COUP-TF I/NR2F1 Antibodies
SKU/Catalog Number
M03833
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-COUP TF1 NR2F1 Monoclonal Antibody catalog # M03833. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COUP TF1 NR2F1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03833)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACHO-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human COUP TF1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03833 is reactive to NR2F1 in Human, Mouse, Rat
Applications
M03833 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
46.156kDa
Background of COUP-TF I/NR2F1
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of COUP TF1 expression in HEK293 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NR2F1 (Source: Uniprot.org, NCBI)
Gene Name
NR2F1
Full Name
COUP transcription factor 1
Weight
46.156kDa
Superfamily
nuclear hormone receptor family
Alternative Names
COUP transcription factor 1; COUPTF I; COUP-TF I; COUP-TF1; COUP-TFI; EAR-3COUP transcription factor I; EAR3COUP-TF I; ERBAL3; ERBAL3V-erbA-related protein 3; NR2F1; Nuclear receptor subfamily 2 group F member 1; nuclear receptor subfamily 2, group F, member 1; SVP44; TCFCOUP1; TFCOUP1NR2F2; transcription factor COUP 1 (chicken ovalbumin upstream promoter 1, v-erb-ahomolog-like 3) NR2F1 BBOAS, BBSOAS, COUP-TFI, COUPTF1, EAR-3, EAR3, ERBAL3, SVP44, TCFCOUP1, TFCOUP1 nuclear receptor subfamily 2 group F member 1 COUP transcription factor 1|COUP transcription factor I|COUP-TF I|COUP-TF1|V-erbA-related protein 3|chicken ovalbumin upstream promoter-transcription factor I|transcription factor COUP 1 (chicken ovalbumin upstream promoter 1, v-erb-a homolog-like 3)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NR2F1, check out the NR2F1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NR2F1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COUP TF1 NR2F1 Monoclonal Antibody (M03833)
Hello CJ!
No publications found for M03833
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-COUP TF1 NR2F1 Monoclonal Antibody
Question
Does anti-COUP TF1 Monoclonal antibody M03833 work on bovine WB with lung uterus?
J. Lewis
Verified customer
Asked: 2020-03-12
Answer
Our lab technicians have not validated anti-COUP TF1 Monoclonal antibody M03833 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-COUP TF1 Monoclonal antibody M03833 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine lung uterus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-12
Question
Is a blocking peptide available for product anti-COUP TF1 Monoclonal antibody (M03833)?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
We do provide the blocking peptide for product anti-COUP TF1 Monoclonal antibody (M03833). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-05
Question
We are currently using anti-COUP TF1 Monoclonal antibody M03833 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
K. Li
Verified customer
Asked: 2015-01-08
Answer
The anti-COUP TF1 Monoclonal antibody (M03833) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-08
