Anti-Homeobox protein DBX2 DBX2 Antibody

Product Name

Anti-Homeobox protein DBX2 DBX2 Antibody

View all DBX2 Antibodies

SKU/Catalog Number

A16753

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Homeobox protein DBX2 DBX2 Antibody (Catalog # A16753). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

DBX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Homeobox protein DBX2 DBX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16753)

Host

Rabbit

Contents

DBX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DBX2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DBX2. The immunogen is located within amino acids 30 - 80 of DBX2.

Cross-reactivity

DBX2 antibody is human specific. At least two isoforms of DBX2 are known to exist; this antibody will only detect the shortest isoform.

Reactive Species

A16753 is reactive to DBX2 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.6 kDa

Background of DBX2

DBX2 is a member of the developing brain homeobox (DBX) protein family, but while the related protein DBX1 is expressed in various regions of the developing brain, DBX2 shows a more restricted pattern of expression in the brain, and is also expressed in some mesenchymal cells such as limb buds and tooth germs. It is thought that DBX1 and DBX2 promote the development of a subset of interneurons, some of which help mediate left-right coordination of locomotor activity. In Xenopus, DBX2 is involved in primary neurogenesis and early neural plate patterning, and is thought to act as a cross-repressive partner of NKX6-2 in the patterning of the ventral neural tube.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A16753 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DBX2 antibody can be used for detection of DBX2 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information DBX2

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