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Product Info Summary
| SKU: | PB9466 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Yes1 Antibody Picoband®
SKU/Catalog Number
PB9466
PB0489 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Yes1 Antibody Picoband® catalog # PB9466. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Yes1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9466)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Yes1, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9466 is reactive to YES1 in Human
Observed Molecular Weight
61 kDa
Calculated molecular weight
60.8 kDa
Background of YES1
Proto-oncogene tyrosine-protein kinase Yes is an enzyme that in humans is encoded by the YES1 gene. This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. YES1 has been shown to interact with Janus kinase 2, CTNND1, RPL10 and Occludin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9466 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human U20S whole cell, human CACO-2 whole cell, human A431 whole cell, human HepG2 whole cell, human MCF-7 whole cell, human PC-3 whole cell
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of YES1 using anti-YES1 antibody (PB9466).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YES1 antigen affinity purified polyclonal antibody (Catalog # PB9466) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YES1 at approximately 61 kDa. The expected band size for YES1 is at 61 kDa.
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IHC analysis of YES1 using anti-YES1 antibody (PB9466).
YES1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-YES1 Antibody (PB9466) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Yes1 Antibody Picoband® (PB9466)
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Customer Q&As
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3 Customer Q&As for Anti-Yes1 Antibody Picoband®
Question
Does anti-Yes1 antibody PB9466 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
According to the expression profile of cervix carcinoma, YES1 is highly expressed in cervix carcinoma. So, it is likely that anti-Yes1 antibody PB9466 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2019-10-17
Question
We are currently using anti-Yes1 antibody PB9466 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
G. Jackson
Verified customer
Asked: 2018-06-07
Answer
The anti-Yes1 antibody (PB9466) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-07
Question
I see that the anti-Yes1 antibody PB9466 works with IHC, what is the protocol used to produce the result images on the product page?
G. Krishna
Verified customer
Asked: 2016-02-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-02-03
