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Product Info Summary
| SKU: | A30467 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-CRBP III Antibody
SKU/Catalog Number
A30467
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CRBP III Antibody (Catalog# A30467). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRBP III Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30467)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CRBP III. Immunogen sequence location: 10-59
Reactive Species
A30467 is reactive to RBP5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
15.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30467 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of LOVO cells, using CRBP III Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CRBP III Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CRBP III Antibody (A30467)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CRBP III Antibody
Question
Is there a BSA free version of anti-CRBP III antibody A30467 available?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CRBP III antibody A30467 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-18
Question
Can you help my question with product A30467, anti-CRBP III antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30467 anti-CRBP III antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-CRBP III antibody A30467. Let me know if you need anything else.
P. Anderson
Verified customer
Asked: 2019-05-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
Does A30467 anti-CRBP III antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Parker
Verified customer
Asked: 2018-06-25
Answer
You can see on the product datasheet, A30467 anti-CRBP III antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-25
Question
Is a blocking peptide available for product anti-CRBP III antibody (A30467)?
A. Wu
Verified customer
Asked: 2017-06-27
Answer
We do provide the blocking peptide for product anti-CRBP III antibody (A30467). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-06-27
Question
Does anti-CRBP III antibody A30467 work for IF with adult mammalian kidney?
T. Carter
Verified customer
Asked: 2016-09-01
Answer
According to the expression profile of adult mammalian kidney, RBP5 is highly expressed in adult mammalian kidney. So, it is likely that anti-CRBP III antibody A30467 will work for IF with adult mammalian kidney.
Boster Scientific Support
Answered: 2016-09-01
Question
We are currently using anti-CRBP III antibody A30467 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
S. Mitchell
Verified customer
Asked: 2014-09-08
Answer
The anti-CRBP III antibody (A30467) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-08
