Anti-CRBP III Antibody

RBP5 antibody

Boster Bio Anti-CRBP III Antibody (Catalog# A30467). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A30467
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC

Product Name

Anti-CRBP III Antibody

View all RBP5 Antibodies

SKU/Catalog Number

A30467

Size

100ul

Form

Liquid

Description

Boster Bio Anti-CRBP III Antibody (Catalog# A30467). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CRBP III Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30467)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human CRBP III. Immunogen sequence location: 10-59

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30467 is reactive to RBP5 in Human, Mouse, Rat

Applications

A30467 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

15.931kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For RBP5 (Source: Uniprot.org, NCBI)

Gene Name

RBP5

Full Name

Retinol-binding protein 5

Weight

15.931kDa

Superfamily

calycin superfamily

Alternative Names

Cellular retinol-binding protein III; CRBP3; CRBPIII; CRBP-III; HRBPiso; putative cellular retinol-binding protein CRBP III; retinol binding protein 5, cellular; retinol-binding protein 5; retinol-binding protein 5, cellular RBP5 CRBP-III, CRBP3, CRBPIII, HRBPiso retinol binding protein 5 retinol-binding protein 5|cellular retinol-binding protein III|putative cellular retinol-binding protein CRBP III|retinol binding protein 5, cellular

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RBP5, check out the RBP5 Infographic

RBP5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RBP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CRBP III Antibody

Question

Is there a BSA free version of anti-CRBP III antibody A30467 available?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CRBP III antibody A30467 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-18

Question

Can you help my question with product A30467, anti-CRBP III antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30467 anti-CRBP III antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-15

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-CRBP III antibody A30467. Let me know if you need anything else.

P. Anderson

Verified customer

Asked: 2019-05-23

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-23

Question

Does A30467 anti-CRBP III antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Parker

Verified customer

Asked: 2018-06-25

Answer

You can see on the product datasheet, A30467 anti-CRBP III antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-06-25

Question

Is a blocking peptide available for product anti-CRBP III antibody (A30467)?

A. Wu

Verified customer

Asked: 2017-06-27

Answer

We do provide the blocking peptide for product anti-CRBP III antibody (A30467). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-27

Question

Does anti-CRBP III antibody A30467 work for IF with adult mammalian kidney?

T. Carter

Verified customer

Asked: 2016-09-01

Answer

According to the expression profile of adult mammalian kidney, RBP5 is highly expressed in adult mammalian kidney. So, it is likely that anti-CRBP III antibody A30467 will work for IF with adult mammalian kidney.

Boster Scientific Support

Answered: 2016-09-01

Question

We are currently using anti-CRBP III antibody A30467 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?

S. Mitchell

Verified customer

Asked: 2014-09-08

Answer

The anti-CRBP III antibody (A30467) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-08

Order DetailsPrice
A30467

100 μL

$335

Free Secondary Antibody

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A30467
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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