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Product Info Summary
| SKU: | A08395-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Hamster, Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-CREBL1 Antibody (C-term)
SKU/Catalog Number
A08395-1
Size
80 µl
Description
Boster Bio Anti-CREBL1 Antibody (C-term) (Catalog # A08395-1). Tested in IHC-P, WB application(s). This antibody reacts with Human, Hamster, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CREBL1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08395-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CREBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 560-589 amino acids from the C-terminal region of human CREBL1.
Reactive Species
A08395-1 is reactive to ATF6B in Hamster, Human, Mouse
Calculated molecular weight
76.7 kDa
Background of ATF6B
CREBL1 is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08395-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2 cell line and human placenta tissue lysate (from left to right), using CREBL1Antibody (C-term) . A08395-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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CREBL1 Antibody (C-term) (Cat. #A08395-1) western blot analysis in mouse NIH-3T3,CHO cell line and mouse cerebellum tissue lysates (35ug/lane). This demonstrates the CREBL1 antibody detected the CREBL1 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. prostate section using CREBL1 Antibody (C-term) (Cat#A08395-1). A08395-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using CREBL1 Antibody (C-term) (Cat#A08395-1). A08395-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Specific Publications For Anti-CREBL1 Antibody (C-term) (A08395-1)
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