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Product Info Summary
| SKU: | A08395-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Hamster, Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-CREBL1 Antibody (C-term)
SKU/Catalog Number
A08395-1
Size
80 µl
Description
Boster Bio Anti-CREBL1 Antibody (C-term) (Catalog # A08395-1). Tested in IHC-P, WB application(s). This antibody reacts with Human, Hamster, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CREBL1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08395-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CREBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 560-589 amino acids from the C-terminal region of human CREBL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08395-1 is reactive to ATF6B in Hamster, Human, Mouse
Applications
A08395-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.709kDa
Background of ATF6 beta
CREBL1 is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysates from Hela, HepG2 cell line and human placenta tissue lysate (from left to right), using CREBL1Antibody (C-term) . A08395-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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CREBL1 Antibody (C-term) (Cat. #A08395-1) western blot analysis in mouse NIH-3T3,CHO cell line and mouse cerebellum tissue lysates (35ug/lane). This demonstrates the CREBL1 antibody detected the CREBL1 protein (arrow).
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Immunohistochemical analysis of paraffin-embedded H. prostate section using CREBL1 Antibody (C-term) (Cat#A08395-1). A08395-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using CREBL1 Antibody (C-term) (Cat#A08395-1). A08395-1 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Protein Target Info & Infographic
Gene/Protein Information For ATF6B (Source: Uniprot.org, NCBI)
Gene Name
ATF6B
Full Name
Cyclic AMP-dependent transcription factor ATF-6 beta
Weight
76.709kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 6 betacAMP-dependent transcription factor ATF-6 beta; ATF6-beta; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; CREBL1CREB-RP; Creb-related protein; Creb-rp; cyclic AMP-dependent transcription factor ATF-6 beta; FLJ10066; G13cAMP-responsive element-binding protein-like 1; Protein G13 ATF6B CREB-RP, CREBL1, G13 activating transcription factor 6 beta cyclic AMP-dependent transcription factor ATF-6 beta|Creb-related protein|cAMP response element-binding protein-related protein|cAMP-dependent transcription factor ATF-6 beta|cAMP-responsive element-binding protein-like 1|protein G13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF6B, check out the ATF6B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF6B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CREBL1 Antibody (C-term) (A08395-1)
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