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Product Info Summary
| SKU: | A06053 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-CRIM1 Antibody
SKU/Catalog Number
A06053
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CRIM1 Antibody (Catalog # A06053). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
CRIM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CRIM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06053)
Host
Rabbit
Contents
CRIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CRIM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Crim1. The immunogen is located within amino acids 910 - 960 of CRIM1.
Cross-reactivity
CRIM1 antibody is human reactive. CRIM1 antibody is predicted to not cross-react with CRIM2.
Reactive Species
A06053 is reactive to CRIM1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
113.7 kDa
Background of CRIM1
CRIM1 (cysteine-rich motor neuron 1), a glycosylated type I transmembrane protein, plays a role in tissue development i.e. capillary formation and maintenance during angiogenesis. It contains an N-terminal IGF-binding protein-like motif and six von Willebrand-like cysteine-rich repeats (CRRs) in its extracellular domain. CRIM1 interacts with BMP4 and BMP7 via the CRRs and functions as an antagonist. CRIM1 is developmentally expressed in a number of tissues including the pancreas, kidney, placenta, brain and blood vessels. CRIM1 may participate in CNS and placental development by interacting with growth factors involved in motor neuron differentiation and survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06053 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CRIM1 antibody can be used for detection of Crim1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CRIM1 in Jurkat cell lysate with Crim1 antibody at 1 μg/mL.
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Immunofluorescence of CRIM1 in Jurkat cells with CRIM1 antibody at 20 μg/mL.
Specific Publications For Anti-CRIM1 Antibody (A06053)
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