Click image to see more details
Product Info Summary
| SKU: | M02860 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CRMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02860
BM5275 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CRMP2 Rabbit Monoclonal Antibody catalog # M02860. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CRMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02860)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACEH-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CRMP2
Reactive Species
M02860 is reactive to DPYSL2 in Human, Mouse, Rat
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02860 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: U87-MG cell, NIH/3T3 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CRMP2 expression in (1) U87-MG cell lysate; (2) NIH/3T3 cell lysate.
Specific Publications For Anti-CRMP2 Rabbit Monoclonal Antibody (M02860)
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7 Customer Q&As for Anti-CRMP2 Rabbit Monoclonal Antibody
Question
We are currently using anti-CRMP2 Monoclonal antibody M02860 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The anti-CRMP2 Monoclonal antibody (M02860) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-09
Question
What sample target on Western Blot application was tested with M02860?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of CRMP2 expression in (1) U87-MG cell lysate; (2) NIH/3T3 cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
Is this M02860 anti-CRMP2 Monoclonal antibody reactive to the isotypes of DPYSL2?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The immunogen of M02860 anti-CRMP2 Monoclonal antibody is A synthesized peptide derived from human CRMP2 Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CRMP2 Monoclonal antibody M02860. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-06
Question
See attached the WB image, lot number and protocol we used for liver using anti-CRMP2 Monoclonal antibody M02860. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-14
Question
Does anti-CRMP2 Monoclonal antibody M02860 work on canine WB with fetal liver?
C. Mangal
Verified customer
Asked: 2015-09-08
Answer
Our lab technicians have not tested anti-CRMP2 Monoclonal antibody M02860 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CRMP2 Monoclonal antibody M02860 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine fetal liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-08
Question
Will M02860 anti-CRMP2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Carter
Verified customer
Asked: 2014-11-04
Answer
You can see on the product datasheet, M02860 anti-CRMP2 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-04
