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Product Info Summary
| SKU: | A05177 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CSH1/Placental Lactogen Antibody
SKU/Catalog Number
A05177
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CSH1/Placental Lactogen Antibody catalog # A05177. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSH1/Placental Lactogen Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05177)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 161-210
Reactive Species
A05177 is reactive to CSH1 in Human
Observed Molecular Weight
39 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05177 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC: 100-300
WB 1:500-2000
ELISA 1:10000-20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Mouse Brain cells using antibody diluted at 1:800.
Specific Publications For Anti-CSH1/Placental Lactogen Antibody (A05177)
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Customer Q&As
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7 Customer Q&As for Anti-CSH1/Placental Lactogen Antibody
Question
I see that the anti-CSH1/Placental Lactogen antibody A05177 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-26
Question
Is this A05177 anti-CSH1/Placental Lactogen antibody reactive to the isotypes of CSH1?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The immunogen of A05177 anti-CSH1/Placental Lactogen antibody is Synthetic peptide from AA range: 161-210. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-05
Question
Does anti-CSH1/Placental Lactogen antibody A05177 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to the expression profile of placenta, CSH1 is highly expressed in placenta. So, it is likely that anti-CSH1/Placental Lactogen antibody A05177 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-10-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CSH1/Placental Lactogen antibody A05177. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-21
Question
My question regarding product A05177, anti-CSH1/Placental Lactogen antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05177 anti-CSH1/Placental Lactogen antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-29
Question
Would A05177 anti-CSH1/Placental Lactogen antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
As indicated on the product datasheet, A05177 anti-CSH1/Placental Lactogen antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-29
Question
Would anti-CSH1/Placental Lactogen antibody A05177 work on goat WB with placenta?
K. Rodriguez
Verified customer
Asked: 2014-03-07
Answer
Our lab technicians have not validated anti-CSH1/Placental Lactogen antibody A05177 on goat. You can run a BLAST between goat and the immunogen sequence of anti-CSH1/Placental Lactogen antibody A05177 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-07
