Product Info Summary
SKU: | A11478 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CSTF1/Cstf 50 Antibody
View all cleavage stimulation factor Antibodies
SKU/Catalog Number
A11478
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CSTF1/Cstf 50 Antibody (Catalog # A11478). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSTF1/Cstf 50 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11478)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CSTF1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11478 is reactive to CSTF1 in Human, Mouse, Rat
Applications
A11478 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.358kDa
Background of cleavage stimulation factor
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
Takagaki Y., Proc. Natl. Acad. Sci. U.S.A. 89:1403-1407(1992).
Deloukas P., Nature 414:865-871(2001).
Kleiman F.E., Science 285:1576-1579(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 293 cells and HUVEC cells, using CSTF1 antibody A11478.
Protein Target Info & Infographic
Gene/Protein Information For CSTF1 (Source: Uniprot.org, NCBI)
Gene Name
CSTF1
Full Name
Cleavage stimulation factor subunit 1
Weight
48.358kDa
Alternative Names
CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; cleavage stimulation factor subunit 1; cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD; cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa; CSTF 50 kDa subunit; CstF-50; CstFp50 CSTF1 CstF-50, CstFp50 cleavage stimulation factor subunit 1 cleavage stimulation factor subunit 1|CF-1 50 kDa subunit|CSTF 50 kDa subunit|cleavage stimulation factor 50 kDa subunit|cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kD|cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSTF1, check out the CSTF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSTF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CSTF1/Cstf 50 Antibody (A11478)
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No publications found for A11478
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CSTF1/Cstf 50 Antibody
Question
Will A11478 anti-CSTF1/Cstf 50 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
As indicated on the product datasheet, A11478 anti-CSTF1/Cstf 50 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
you antibody to test anti-CSTF1/Cstf 50 antibody A11478 on rat testis for research purposes, then I may be interested in using anti-CSTF1/Cstf 50 antibody A11478 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The products we sell, including anti-CSTF1/Cstf 50 antibody A11478, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-CSTF1/Cstf 50 antibody A11478 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The anti-CSTF1/Cstf 50 antibody (A11478) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-30
Question
Do you have a BSA free version of anti-CSTF1/Cstf 50 antibody A11478 available?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CSTF1/Cstf 50 antibody A11478 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-27
Question
I was wanting to use your anti-CSTF1/Cstf 50 antibody for WB for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?
N. Wu
Verified customer
Asked: 2019-01-14
Answer
It shows on the product datasheet, A11478 anti-CSTF1/Cstf 50 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-14
Question
Here is the WB image, lot number and protocol we used for testis using anti-CSTF1/Cstf 50 antibody A11478. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CSTF1/Cstf 50 antibody A11478. Let me know if you need anything else.
T. Parker
Verified customer
Asked: 2015-01-07
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-01-07