Anti-Ctip2/BCL11B Antibody Picoband™

Product Name

Anti-Ctip2/BCL11B Antibody Picoband™

View all Bcl11b Antibodies

SKU/Catalog Number

A01485-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ctip2/BCL11B Antibody Picoband™ catalog # A01485-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Ctip2/BCL11B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01485-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Ctip2/BCL11B recombinant protein (Position: K838-S894).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01485-1 is reactive to Bcl11b in Human, Mouse, Rat

Applications

A01485-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

94.566kDa

Background of Bcl11b

B-cell lymphoma/leukemia 11B is a protein that in humans is encoded by the BCL11B gene. This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Ctip2/BCL11B using anti-Ctip2/BCL11B antibody (A01485-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human MOLT-4 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates,
Lane 6: mouse Ana-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ctip2/BCL11B antigen affinity purified polyclonal antibody (Catalog # A01485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ctip2/BCL11B at approximately 130 kDa. The expected band size for Ctip2/BCL11B is at 96 kDa.

Gene/Protein Information For Bcl11b (Source: Uniprot.org, NCBI)

Alternative Names

B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B; B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; BCL-11B; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; CTIP-2; CTIP2ZNF856B; hRit1; hRIT1-alpha; Radiation-induced tumor suppressor gene 1 protein; RIT1; zinc finger protein hRit1 alpha Bcl11b|9130430L19Rik, AI604821, B630002E05Rik, BCL-11B, CTI, Ctip2, Ri, Rit1|B cell leukemia/lymphoma 11B|B-cell lymphoma/leukemia 11B|B-cell CLL/lymphoma 11B|B-cell lymphoma/leukaemia 11B|COUP-TF-interacting protein 2|mRit1|radiation-induced tumor suppressor gene 1 protein

For more info on Bcl11b, check out the Bcl11b Infographic

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In this infographic, you will see the following information for Bcl11b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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