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Product Info Summary
| SKU: | M02550 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RNA Helicase A Rabbit Monoclonal Antibody
SKU/Catalog Number
M02550
BM5580 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RNA Helicase A Rabbit Monoclonal Antibody catalog # M02550. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNA Helicase A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02550)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEEH-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RNA Helicase A
Reactive Species
M02550 is reactive to DHX9 in Human, Mouse, Rat
Observed Molecular Weight
141 kDa
Calculated molecular weight
141.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02550 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human Jurkat whole cell, human Hela whole cell, human 293T whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of RNA Helicase A using anti-RNA Helicase A antibody (M02550).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNA Helicase A antigen affinity purified monoclonal antibody (Catalog # M02550) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNA Helicase A at approximately 141 kDa. The expected band size for RNA Helicase A is at 141 kDa.
Specific Publications For Anti-RNA Helicase A Rabbit Monoclonal Antibody (M02550)
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Customer Q&As
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7 Customer Q&As for Anti-RNA Helicase A Rabbit Monoclonal Antibody
Question
you antibody to test anti-RNA Helicase A Monoclonal antibody M02550 on mouse liver for research purposes, then I may be interested in using anti-RNA Helicase A Monoclonal antibody M02550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The products we sell, including anti-RNA Helicase A Monoclonal antibody M02550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-13
Question
Does anti-RNA Helicase A Monoclonal antibody M02550 work for IF with liver?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
According to the expression profile of liver, DHX9 is highly expressed in liver. So, it is likely that anti-RNA Helicase A Monoclonal antibody M02550 will work for IF with liver.
Boster Scientific Support
Answered: 2020-02-26
Question
Does anti-RNA Helicase A Monoclonal antibody M02550 work on goat IF with embryonic kidney?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
Our lab technicians have not validated anti-RNA Helicase A Monoclonal antibody M02550 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RNA Helicase A Monoclonal antibody M02550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat embryonic kidney in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-19
Question
What sample target on Western Blot application was tested with M02550?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of RNA Helicase A expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
We are currently using anti-RNA Helicase A Monoclonal antibody M02550 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
The anti-RNA Helicase A Monoclonal antibody (M02550) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-RNA Helicase A Monoclonal antibody M02550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-19
Question
Is a blocking peptide available for product anti-RNA Helicase A Monoclonal antibody (M02550)?
B. Brown
Verified customer
Asked: 2019-02-11
Answer
We do provide the blocking peptide for product anti-RNA Helicase A Monoclonal antibody (M02550). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-11
