Anti-RNA Helicase A Rabbit Monoclonal Antibody

RNA Helicase A antibody

Boster Bio Anti-RNA Helicase A Rabbit Monoclonal Antibody catalog # M02550. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M02550
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-RNA Helicase A Rabbit Monoclonal Antibody

View all RNA Helicase A Antibodies

SKU/Catalog Number

M02550

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-RNA Helicase A Rabbit Monoclonal Antibody catalog # M02550. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNA Helicase A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02550)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEEH-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human RNA Helicase A

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02550 is reactive to DHX9 in Human, Mouse, Rat

Applications

M02550 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

140.958kDa

Background of RNA Helicase A

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:20

Validation Images & Assay Conditions

Gene/Protein Information For DHX9 (Source: Uniprot.org, NCBI)

Gene Name

DHX9

Full Name

ATP-dependent RNA helicase A

Weight

140.958kDa

Superfamily

DEAD box helicase family

Alternative Names

DDX9ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; EC 3.6.1; EC 3.6.1.15; EC 3.6.4.13; FLJ17406; leukophysin; LKP; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA DHX9 DDX9, LKP, NDH2, NDHII, RHA DExH-box helicase 9 ATP-dependent RNA helicase A|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9|DEAH (Asp-Glu-Ala-His) box helicase 9|DEAH (Asp-Glu-Ala-His) box polypeptide 9|DEAH box protein 9|DEAH-box helicase 9|RNA helicase A|leukophysin|nuclear DNA helicase II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DHX9, check out the DHX9 Infographic

DHX9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DHX9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-RNA Helicase A Rabbit Monoclonal Antibody

Question

you antibody to test anti-RNA Helicase A Monoclonal antibody M02550 on mouse liver for research purposes, then I may be interested in using anti-RNA Helicase A Monoclonal antibody M02550 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The products we sell, including anti-RNA Helicase A Monoclonal antibody M02550, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-13

Question

Does anti-RNA Helicase A Monoclonal antibody M02550 work for IF with liver?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

According to the expression profile of liver, DHX9 is highly expressed in liver. So, it is likely that anti-RNA Helicase A Monoclonal antibody M02550 will work for IF with liver.

Boster Scientific Support

Answered: 2020-02-26

Question

Does anti-RNA Helicase A Monoclonal antibody M02550 work on goat IF with embryonic kidney?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

Our lab technicians have not validated anti-RNA Helicase A Monoclonal antibody M02550 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RNA Helicase A Monoclonal antibody M02550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat embryonic kidney in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-19

Question

What sample target on Western Blot application was tested with M02550?

Verified customer

Asked: 2020-01-03

Answer

Western blot analysis of RNA Helicase A expression in Jurkat cell lysate.

Boster Scientific Support

Answered: 2020-01-16

Question

We are currently using anti-RNA Helicase A Monoclonal antibody M02550 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

The anti-RNA Helicase A Monoclonal antibody (M02550) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-07

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-RNA Helicase A Monoclonal antibody M02550. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-19

Question

Is a blocking peptide available for product anti-RNA Helicase A Monoclonal antibody (M02550)?

B. Brown

Verified customer

Asked: 2019-02-11

Answer

We do provide the blocking peptide for product anti-RNA Helicase A Monoclonal antibody (M02550). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-11

Order DetailsPrice
M02550

100μL

$370
M02550-30ul

30μL sample

$99
M02550-carrier-free

Carrier Free

$370

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M02550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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