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Product Info Summary
| SKU: | M00020 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CTLA4 (CD152) Monoclonal Antibody
SKU/Catalog Number
M00020
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CTLA4 (CD152) Monoclonal Antibody catalog # M00020. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTLA4 (CD152) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00020)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADCD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CTLA4 (CD152)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00020 is reactive to CTLA4 in Human, Mouse, Rat
Applications
M00020 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
24.656kDa
Background of CTLA-4
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CTLA4 (CD152) expression in NIH/3T3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CTLA4 (Source: Uniprot.org, NCBI)
Gene Name
CTLA4
Full Name
Cytotoxic T-lymphocyte protein 4
Weight
24.656kDa
Alternative Names
CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 CTLA4 ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 cytotoxic T-lymphocyte associated protein 4 cytotoxic T-lymphocyte protein 4|celiac disease 3|cytotoxic T lymphocyte associated antigen 4 short spliced form|cytotoxic T-lymphocyte-associated serine esterase-4|insulin-dependent diabetes mellitus 12|ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTLA4, check out the CTLA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTLA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTLA4 (CD152) Monoclonal Antibody (M00020)
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No publications found for M00020
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-CTLA4 (CD152) Monoclonal Antibody
Question
Would M00020 anti-CTLA4 (CD152) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
It shows on the product datasheet, M00020 anti-CTLA4 (CD152) Monoclonal antibody as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-13
Question
Is there a BSA free version of anti-CTLA4 (CD152) Monoclonal antibody M00020 available?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CTLA4 (CD152) Monoclonal antibody M00020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-02
Question
Will anti-CTLA4 (CD152) Monoclonal antibody M00020 work for ICC with lymphocyte?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
According to the expression profile of lymphocyte, CTLA4 is highly expressed in lymphocyte. So, it is likely that anti-CTLA4 (CD152) Monoclonal antibody M00020 will work for ICC with lymphocyte.
Boster Scientific Support
Answered: 2019-08-01
Question
I have a question about product M00020, anti-CTLA4 (CD152) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00020 anti-CTLA4 (CD152) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
We are currently using anti-CTLA4 (CD152) Monoclonal antibody M00020 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
The anti-CTLA4 (CD152) Monoclonal antibody (M00020) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-22
Question
We purchased anti-CTLA4 (CD152) Monoclonal antibody for ICC on lymph node in a previous project. I am using human, and I plan to use the antibody for IF next. We need examining lymph node as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
I have checked the website and datasheets of our anti-CTLA4 (CD152) Monoclonal antibody and it seems that M00020 has been validated on human in both ICC and IF. Thus M00020 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-10
Question
I have attached the WB image, lot number and protocol we used for lymphocyte using anti-CTLA4 (CD152) Monoclonal antibody M00020. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
I see that the anti-CTLA4 (CD152) Monoclonal antibody M00020 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-30
Question
Is this M00020 anti-CTLA4 (CD152) Monoclonal antibody reactive to the isotypes of CTLA4?
J. Taylor
Verified customer
Asked: 2019-02-26
Answer
The immunogen of M00020 anti-CTLA4 (CD152) Monoclonal antibody is A synthesized peptide derived from human CTLA4 (CD152). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-26
Question
I was wanting to use your anti-CTLA4 (CD152) Monoclonal antibody for ICC for human lymphocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lymphocyte identification?
P. Krishna
Verified customer
Asked: 2019-02-12
Answer
As indicated on the product datasheet, M00020 anti-CTLA4 (CD152) Monoclonal antibody has been validated for IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-CTLA4 (CD152) Monoclonal antibody M00020. Let me know if you need anything else.
J. Patel
Verified customer
Asked: 2018-12-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-28
Question
We have seen staining in rat lymph node. Are there any suggestions? Is anti-CTLA4 (CD152) Monoclonal antibody supposed to stain lymph node positively?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
According to literature lymph node does express CTLA4. According to Uniprot.org, CTLA4 is expressed in lymph node, lung, lymphocyte, among other tissues. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2018-05-30
Question
My lab would like to test anti-CTLA4 (CD152) Monoclonal antibody M00020 on human lymphocyte for research purposes, then I may be interested in using anti-CTLA4 (CD152) Monoclonal antibody M00020 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Jones
Verified customer
Asked: 2017-12-20
Answer
The products we sell, including anti-CTLA4 (CD152) Monoclonal antibody M00020, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-20
Question
Is a blocking peptide available for product anti-CTLA4 (CD152) Monoclonal antibody (M00020)?
T. Jackson
Verified customer
Asked: 2015-09-08
Answer
We do provide the blocking peptide for product anti-CTLA4 (CD152) Monoclonal antibody (M00020). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-09-08
Question
We were content with the WB result of your anti-CTLA4 (CD152) Monoclonal antibody. However we have seen positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is CTLA4 supposed to be expressed?
B. Edwards
Verified customer
Asked: 2013-09-19
Answer
From what I have seen in literature, lung does express CTLA4. Generally CTLA4 expresses in cell membrane. Regarding which tissues have CTLA4 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334
Lymph node, Pubmed ID: 10493833
Lymphocyte, Pubmed ID: 3220103
Boster Scientific Support
Answered: 2013-09-19
