Product Info Summary
| SKU: | A12414-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CTRP6 C1QTNF6 Antibody
SKU/Catalog Number
A12414-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CTRP6 C1QTNF6 Antibody (Catalog # A12414-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CTRP6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CTRP6 C1QTNF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12414-1)
Host
Rabbit
Contents
CTRP6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CTRP6 antibody was raised against a 15 amino acid synthetic peptide from near the center of human CTRP6. The immunogen is located within amino acids 80 - 130 of CTRP6.
Cross-reactivity
These proteins often migrate in SDS-PAGE at positions other than their predicted size.
Reactive Species
A12414-1 is reactive to C1QTNF6 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.9 kDa
Background of C1QTNF6
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. CTRP6 contains at least 4 glycosylation motifs, suggesting that CTRP6 may be highly post-translationally modified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12414-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CTRP6 antibody can be used for the detection of CTRP6 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CTRP6 in mouse brain cell lysate with CTRP6 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
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Immunohistochemistry of CTRP6 in human brain tissue with CTRP6 antibody at 10 μg/mL.
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Immunofluorescence of CTRP6 in Human Brain cells with CTRP6 antibody at 20 μg/mL.
Specific Publications For Anti-CTRP6 C1QTNF6 Antibody (A12414-1)
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