Click image to see more details
Product Info Summary
| SKU: | M00747 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Cullin 3 Monoclonal Antibody
SKU/Catalog Number
M00747
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cullin 3 Monoclonal Antibody catalog # M00747. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cullin 3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00747)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEOD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cullin 3 Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00747 is reactive to CUL3 in Human, Mouse, Rat
Applications
M00747 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Observed Molecular Weight
133 kDa
Calculated molecular weight
88.93kDa
Background of Cullin 3
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:50
FC 1:60
Validation Images & Assay Conditions
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Western blot analysis of Cullin 3 expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CUL3 (Source: Uniprot.org, NCBI)
Gene Name
CUL3
Full Name
Cullin-3
Weight
88.93kDa
Superfamily
cullin family
Alternative Names
CUL-3; cullin 3; cullin-3; KIAA0617 CUL3 CUL-3, PHA2E cullin 3 cullin-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CUL3, check out the CUL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CUL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cullin 3 Monoclonal Antibody (M00747)
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No publications found for M00747
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Cullin 3 Monoclonal Antibody
Question
Will anti-Cullin 3 Monoclonal antibody M00747 work on canine WB with sperm?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Our lab technicians have not tested anti-Cullin 3 Monoclonal antibody M00747 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Cullin 3 Monoclonal antibody M00747 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine sperm in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin uterus using anti-Cullin 3 Monoclonal antibody M00747. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
I see that the anti-Cullin 3 Monoclonal antibody M00747 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-Cullin 3 Monoclonal antibody (M00747)?
Verified Customer
Verified customer
Asked: 2018-01-24
Answer
We do provide the blocking peptide for product anti-Cullin 3 Monoclonal antibody (M00747). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-24
Question
Can you help my question with product M00747, anti-Cullin 3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00747 anti-Cullin 3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-13
Question
Is this M00747 anti-Cullin 3 Monoclonal antibody reactive to the isotypes of CUL3?
B. Anderson
Verified customer
Asked: 2014-08-18
Answer
The immunogen of M00747 anti-Cullin 3 Monoclonal antibody is A synthesized peptide derived from human Cullin 3 Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-08-18
Question
Our lab want to know about to test anti-Cullin 3 Monoclonal antibody M00747 on human skin uterus for research purposes, then I may be interested in using anti-Cullin 3 Monoclonal antibody M00747 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Williams
Verified customer
Asked: 2014-06-20
Answer
The products we sell, including anti-Cullin 3 Monoclonal antibody M00747, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-20
