Anti-Cullin4A Monoclonal Antibody

Cullin 4a antibody

Boster Bio Anti-Cullin4A Monoclonal Antibody catalog # M01579. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M01579
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-Cullin4A Monoclonal Antibody

View all Cullin 4a Antibodies

SKU/Catalog Number

M01579

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Cullin4A Monoclonal Antibody catalog # M01579. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cullin4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01579)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEIH-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Cullin4A Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01579 is reactive to CUL4A in Human

Applications

M01579 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

87.68kDa

Background of Cullin 4a

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CUL4A (Source: Uniprot.org, NCBI)

Gene Name

CUL4A

Full Name

Cullin-4A

Weight

87.68kDa

Superfamily

cullin family

Alternative Names

CUL4A; CUL-4A; Cullin 4a; cullin-4A CUL4A cullin 4A cullin-4A|CUL-4A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CUL4A, check out the CUL4A Infographic

CUL4A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CUL4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Cullin4A Monoclonal Antibody

Question

Would anti-Cullin4A Monoclonal antibody M01579 work for IF with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

According to the expression profile of cervix carcinoma, CUL4A is highly expressed in cervix carcinoma. So, it is likely that anti-Cullin4A Monoclonal antibody M01579 will work for IF with cervix carcinoma.

Boster Scientific Support

Answered: 2020-01-23

Question

I see that the anti-Cullin4A Monoclonal antibody M01579 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-13

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Cullin4A Monoclonal antibody M01579. Let me know if you need anything else.

J. Zhang

Verified customer

Asked: 2019-03-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-22

Question

Does anti-Cullin4A Monoclonal antibody M01579 work on dog IP with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

Our lab technicians have not validated anti-Cullin4A Monoclonal antibody M01579 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Cullin4A Monoclonal antibody M01579 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-11

Order DetailsPrice
M01579

100μL

$370
M01579-30ul

30μL sample

$99
M01579-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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