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Product Info Summary
| SKU: | M01579 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cullin4A Monoclonal Antibody
SKU/Catalog Number
M01579
BM5633 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cullin4A Monoclonal Antibody catalog # M01579. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cullin4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01579)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cullin4A Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.
Reactive Species
M01579 is reactive to CUL4A in Human
Observed Molecular Weight
88 kDa
Calculated molecular weight
87.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01579 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Cullin4A using anti-Cullin4A antibody (M01579).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin4A antigen affinity purified monoclonal antibody (Catalog # M01579) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin4A at approximately 88 kDa. The expected band size for Cullin4A is at 88 kDa.
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IHC analysis of CUL4A using anti-CUL4A antibody (M01579).
CUL4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL4A Antibody (M01579) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of CUL4A using anti-CUL4A antibody (M01579).
CUL4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CUL4A Antibody (M01579) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Cullin4A Monoclonal Antibody (M01579)
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4 Customer Q&As for Anti-Cullin4A Monoclonal Antibody
Question
Would anti-Cullin4A Monoclonal antibody M01579 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
According to the expression profile of cervix carcinoma, CUL4A is highly expressed in cervix carcinoma. So, it is likely that anti-Cullin4A Monoclonal antibody M01579 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2020-01-23
Question
I see that the anti-Cullin4A Monoclonal antibody M01579 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-09-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Cullin4A Monoclonal antibody M01579. Let me know if you need anything else.
J. Zhang
Verified customer
Asked: 2019-03-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-22
Question
Does anti-Cullin4A Monoclonal antibody M01579 work on dog IP with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
Our lab technicians have not validated anti-Cullin4A Monoclonal antibody M01579 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Cullin4A Monoclonal antibody M01579 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-11
