CXCL12 antibody (A00053-1) performs well in IHC, WB and shows reactivity with Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 2 Publications
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a00053 1 cxcl12 primary antibodies wb testing 1a00053 1 cxcl12 primary antibodies ihc testing 2

Anti-CXCL12 Antibody Picoband®

CXCL12 antibody

Reactive SpeciesHostApplication
Mouse, RatRabbitIHC, WB

SKU: A00053-1

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Guarantee / Product Promise

Tested Applications

CXCL12 antibody was positively detected by WB in:

rat stomach tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CXCL12 antibody was positively detected by IHC in:

mouse spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
Rat
Tested
Mouse
Tested
Recommend
Human
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-CXCL12 Antibody Picoband®
View all CXCL12 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse CXCL12, which shares 96.2% and 95.5% amino acid (aa) sequence identity with human and rat CXCL12, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
11 kDa
Calculated Molecular Weight

10.7 kDa

Background of CXCL12
The stromal cell-derived factor 1 (SDF1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that in humans is encoded by the CXCL12 gene on chromosome 10. This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-CXCL12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00053-1)

Protocols

Western Blot protocol for CXCL12 antibody
Immunohistochemistry (IHC) protocol for CXCL12 antibody

Publications

Application:
Species:
Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration
Published: 2018-07-31
Journal: ACS Applied Materials & Interfaces
Application: IHC
Impact Factor: 8.10
Species: Rat
PMID: 30063134
Crucial Role of SDF-1/CXCR4 Interaction in the Recruitment of Transplanted Dermal Multipotent Cells to Sublethally Irradiated Bone Marrow
Published: 2006-09-15
Journal: JOURNAL OF RADIATION RESEARCH
Application: IHC
Impact Factor: 1.36
Species: Rat
PMID: 16974072

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Product Promise

We promise Anti-CXCL12 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
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A00053-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 9, 2026