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- Table of Contents
CXCR1 antibody (PA2080) performs well in Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Flow Cytometry analysis of human PBMC cells using anti-CXCR1 antibody (PA2080).
Overlay histogram showing human PBMC cells stained with PA2080 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR1 Antibody (PA2080, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing human PBMC cells stained with PA2080 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR1 Antibody (PA2080, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CXCR1 Antibody Picoband®
CXCR1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, WB |
Tested Applications
CXCR1 antibody was positively detected by WB in:
human Jurkat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CXCR1 antibody was positively detected by FC in:
human PBMC cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CXCR1 Antibody Picoband®
View all CXCR1 Antibodies
View all CXCR1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
39.8 kDa
Background of CXCR1
CXCR1 (Chemokine,CXC Motif, Receptor 1), also known as IL8RA or IL8R1, is a chemokine receptor. IL8RA is its human gene. Morris et al. (1992) mapped the IL8RA gene to chromosome 2q35. Hartl et al. (2007) concluded that CXCR1 cleavage and its functional consequences represent an important pathophysiologic mechanism in CF and other neutrophilic airway diseases. Receptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
Cite This Product
Anti-CXCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2080)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
80%
Moderate Homology
Cow
73%
Moderate Homology
Sheep
73%
Moderate Homology
Dog
67%
Low homology
Rabbit
60%
Low homology
Rat
40%
Low homology
Mouse
33%
Low homology
Zebrafish
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CXCR1 antibody
Flow Cytometry (FC) protocol for CXCR1 antibody
Publications
Application:
Species:
Activation of CXCL6/CXCR1/2 Axis Promotes the Growth and Metastasis of Osteosarcoma Cells in vitro and in vivo
Published: 2019-03-28
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 3.85
Species: Mouse,Human
PMID: 30984000
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Product Promise
We promise Anti-CXCR1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2080
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026