Click image to see more details
Product Info Summary
| SKU: | M02656 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02656
BM4525 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody catalog # M02656. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02656)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FEI-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CXCR7
Reactive Species
M02656 is reactive to ACKR3 in Human, Mouse, Rat
Observed Molecular Weight
38 kDa
Calculated molecular weight
41.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02656 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
FC 1:20
Positive Control
WB: human RT4 whole cell, human Hela whole cell, human A431 whole cell, human MCF-7 whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CXCR7/ACKR3 using anti-CXCR7/ACKR3 antibody (M02656).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR7/ACKR3 antigen affinity purified monoclonal antibody (M02656) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CXCR7/ACKR3 at approximately 38 kDa. The expected band size for CXCR7/ACKR3 is at 41 kDa.
Specific Publications For Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody (M02656)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-CXCR7 ACKR3 Rabbit Monoclonal Antibody
Question
Would anti-CXCR7 Rabbit Monoclonal antibody M02656 work for WB with synovial joint?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
According to the expression profile of synovial joint, ACKR3 is highly expressed in synovial joint. So, it is likely that anti-CXCR7 Rabbit Monoclonal antibody M02656 will work for WB with synovial joint.
Boster Scientific Support
Answered: 2020-04-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for synovial joint using anti-CXCR7 Rabbit Monoclonal antibody M02656. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-21
Question
We are currently using anti-CXCR7 Rabbit Monoclonal antibody M02656 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
S. Bhatt
Verified customer
Asked: 2019-08-16
Answer
The anti-CXCR7 Rabbit Monoclonal antibody (M02656) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-16
