Product Info Summary
SKU: | A03487 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-CYB5R3 (I169) Antibody
SKU/Catalog Number
A03487
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CYB5R3 (I169) Antibody catalog # A03487. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYB5R3 (I169) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03487)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Casein Kinase IIbeta around the phosphorylation site of S209.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03487 is reactive to CYB5R3 in Human, Mouse, Rat
Applications
A03487 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.235kDa
Background of CYB5R3
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. May be involved in the remodeling of the actin cytoskeleton. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activating protein on Rho.
Wirth J.A., J. Cell Sci. 109:653-661(1996).
Grewal P.K., Gene 240:389-398(1999).
Post P.L., J. Cell Sci. 111:941-950(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of CYB5R3 using Anti-CYB5R3 (I169) Antibody (A03487).
Immunohistochemistry (IHC) analyzes of CYB5R3 (I169) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-CYB5R3 (I169) Antibody A03487
Western blot (WB) analysis of CYB5R3 (I169) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate(40ug)
Lane2:A549 whole cell lysate(40ug)
Lane3:The Testis tissue lysate of Rat(40ug)
Lane4:The Testis tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYB5R3 (Source: Uniprot.org, NCBI)
Gene Name
CYB5R3
Full Name
NADH-cytochrome b5 reductase 3
Weight
34.235kDa
Superfamily
flavoprotein pyridine nucleotide cytochrome reductase family
Alternative Names
B5RNADH-cytochrome b5 reductase 3; cytochrome b5 reductase 3; Cytochrome b5 reductase; DIA1NADH-cytochrome b5 reductase 3 membrane-bound form; diaphorase (NADH) (cytochrome b-5 reductase); diaphorase-1; EC 1.6.2.2; NADH-cytochrome b5 reductase 3 soluble form CYB5R3 B5R, DIA1 cytochrome b5 reductase 3 NADH-cytochrome b5 reductase 3|NADH-cytochrome b5 reductase 3 membrane-bound form|NADH-cytochrome b5 reductase 3 soluble form|diaphorase-1|mutant NADH-cytochrome b5 reductase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYB5R3, check out the CYB5R3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYB5R3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYB5R3 (I169) Antibody (A03487)
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No publications found for A03487
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Customer Reviews
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