Click image to see more details
Product Info Summary
| SKU: | M00328 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00328
BM4576 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00328)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GOH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CYBB
Reactive Species
M00328 is reactive to CYBB in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
65.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00328 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB:human MCF-7 whole cell, human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of NOX2 using anti-NOX2 antibody (M00328).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2 antigen affinity purified monoclonal antibody (M00328) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOX2 at approximately 65 kDa. The expected band size for NOX2 is at 65 kDa.
Specific Publications For Anti-CYBB/Nox2 Rabbit Monoclonal Antibody (M00328)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CYBB/Nox2 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
Question
Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Our lab technicians have not tested anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Is there a BSA free version of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work for WB with liver?
S. Roberts
Verified customer
Asked: 2018-08-31
Answer
According to the expression profile of liver, CYBB is highly expressed in liver. So, it is likely that anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 will work for WB with liver.
Boster Scientific Support
Answered: 2018-08-31
Question
I see that the anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-15
Question
Is a blocking peptide available for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328)?
J. Carter
Verified customer
Asked: 2018-04-06
Answer
We do provide the blocking peptide for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-04-06
