Anti-CYBB/Nox2 Rabbit Monoclonal Antibody

CYBB/NOX2 antibody

Boster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: M00328
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-CYBB/Nox2 Rabbit Monoclonal Antibody

View all CYBB/NOX2 Antibodies

SKU/Catalog Number

M00328

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYBB/Nox2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00328)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GOH-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CYBB

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00328 is reactive to CYBB in Human, Mouse, Rat

Applications

M00328 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

47 kDa

Calculated molecular weight

65.336kDa

Background of CYBB/NOX2

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For CYBB (Source: Uniprot.org, NCBI)

Gene Name

CYBB

Full Name

Cytochrome b-245 heavy chain

Weight

65.336kDa

Alternative Names

CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYBB, check out the CYBB Infographic

CYBB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00328 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
M00328 usage in article: APP:WB, SAMPLE:KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA

Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation

Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity

Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?

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6 Customer Q&As for Anti-CYBB/Nox2 Rabbit Monoclonal Antibody

Question

Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work on pig WB with liver?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

Our lab technicians have not tested anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-23

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-27

Question

Is there a BSA free version of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 available?

Verified Customer

Verified customer

Asked: 2019-01-23

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-23

Question

Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work for WB with liver?

S. Roberts

Verified customer

Asked: 2018-08-31

Answer

According to the expression profile of liver, CYBB is highly expressed in liver. So, it is likely that anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 will work for WB with liver.

Boster Scientific Support

Answered: 2018-08-31

Question

I see that the anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-15

Question

Is a blocking peptide available for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328)?

J. Carter

Verified customer

Asked: 2018-04-06

Answer

We do provide the blocking peptide for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-06

Order DetailsPrice
M00328

100μL

$370
M00328-30ul

30μL sample

$99
M00328-carrier-free

Carrier Free

$370

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M00328
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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