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- Table of Contents
CCNB1 antibody (M00745-3) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cyclin B1 Antibody.
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Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
CCNB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
CCNB1 antibody was positively detected by IHC in:
human breast cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Recommend
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
View all CCNB1 Antibodies
View all CCNB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin B1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
48.3 kDa
Cite This Product
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-3)
Protocols
Immunohistochemistry (IHC) protocol for CCNB1 antibody
Publications
Application:
Species:
Zhang H,Su Y,Wang J,Gao Y,Yang F,Li G,Shi Q.Ginsenoside Rb1 promotes the growth of mink hair follicle via PI3K/AKT/GSK-3β signaling pathway.Life Sci.2019 Jul 15;229:210-218.doi:10.1016/j.lfs.2019.05.033.Epub 2019 May 16.PMID:31102746.
Species: American Minks
PMID: 31102746
Li K, Zhu Zc, Liu Yj, Liu Jw, Wang Ht, Xiong Zq, Shen X, Hu Zl, Zheng J. Int J Clin Exp Pathol. 2013 Sep 15;6(11):2460-7. Ecollection 2013. Zfx Knockdown Inhibits Growth And Migration Of Non-Small Cell Lung Carcinoma Cell Line H1299.
PMID: 24228108
Yang H, Gu J, Zheng Q, Li M, Lian X, Miao J, Jiang J, Wei W. J Biol Chem. 2011 Apr 1;286(13):11865-74. Doi: 10.1074/Jbc.M110.136929. Epub 2011 Feb 10. Rpb5-Mediating Protein Is Required For The Proliferation Of Hepatocellular Carcinoma Cells.
PMID: 21310960
Review
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Product Promise
We promise Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00745-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026