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Product Info Summary
| SKU: | M00745-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00745-3
BM4251 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody catalog # M00745-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00745-3)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CIC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin B1
Reactive Species
M00745-3 is reactive to CCNB1 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00745-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Positive Control
IHC: human breast cancer tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:6K dilution for 1 hour at room temperature.
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Western blot analysis of Cyclin B1 expression in (1)HaCaT cell lysates; (2)HepG2 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cyclin B1 Antibody.
Specific Publications For Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody (M00745-3)
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Customer Q&As
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3 Customer Q&As for Anti-Cyclin B1 CCNB1 Rabbit Monoclonal Antibody
Question
Is this M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody reactive to the isotypes of CCNB1?
Verified Customer
Verified customer
Asked: 2018-11-13
Answer
The immunogen of M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Cyclin B1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-13
Question
I was wanting to use your anti-Cyclin B1 Rabbit Monoclonal antibody for IP for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-06-04
Answer
It shows on the product datasheet, M00745-3 anti-Cyclin B1 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-04
Question
We are interested in to test anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3 on human cervix carcinoma for research purposes, then I may be interested in using anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Edwards
Verified customer
Asked: 2015-09-18
Answer
The products we sell, including anti-Cyclin B1 Rabbit Monoclonal antibody M00745-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-18
