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Product Info Summary
| SKU: | A03766-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband®
SKU/Catalog Number
A03766-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband® catalog # A03766-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03766-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP11B1/C11B2/CYP11B2 recombinant protein (Position: M1-P491).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03766-3 is reactive to CYP11B1/2 in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
57.6 kDa
Background of CYP11B1/2
CYP11B1 shows close homology to the CYP11B2 gene, which encodes aldosterone synthase and is normally expressed only in the zona glomerulosa. Both genes map to chromosome 8q21. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03766-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5μg/ml | Mouse, Rat |
| Flow Cytometry (Fixed) | 1-3μg/1x106 cells | Human |
| ELISA | 0.1-0.5μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of CYP11B1/C11B2/CYP11B2 using anti-CYP11B1/C11B2/CYP11B2 antibody (A03766-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 antigen affinity purified polyclonal antibody (Catalog # A03766-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP11B1/C11B2/CYP11B2 at approximately 55KD. The expected band size for CYP11B1/C11B2/CYP11B2 is at 55KD.
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Flow Cytometry analysis of 293T cells using anti-CYP11B1/C11B2/CYP11B2 antibody (A03766-3).
Overlay histogram showing 293T cells stained with A03766-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 Antibody (A03766-3,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband® (A03766-3)
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