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Product Info Summary
| SKU: | A10507 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 2A7 CYP2A7 Antibody
SKU/Catalog Number
A10507
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2A7 CYP2A7 Antibody catalog # A10507. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2A7 CYP2A7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10507)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human CYP2A7.
Reactive Species
A10507 is reactive to CYP2A7 in Human
Calculated molecular weight
56.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10507 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of 22RV1 cells using CYP2A7 Polyclonal Antibody diluted at 1:2000
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Cytochrome P450 2A7 CYP2A7 Antibody (A10507)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Cytochrome P450 2A7 CYP2A7 Antibody
Question
I see that the anti-CYP2A7 antibody A10507 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-03
Question
We are currently using anti-CYP2A7 antibody A10507 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
The anti-CYP2A7 antibody (A10507) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-02
Question
Does A10507 anti-CYP2A7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
It shows on the product datasheet, A10507 anti-CYP2A7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-19
Question
Is a blocking peptide available for product anti-CYP2A7 antibody (A10507)?
P. Li
Verified customer
Asked: 2015-10-30
Answer
We do provide the blocking peptide for product anti-CYP2A7 antibody (A10507). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-10-30
Question
Do you have a BSA free version of anti-CYP2A7 antibody A10507 available?
J. Collins
Verified customer
Asked: 2013-12-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CYP2A7 antibody A10507 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-12-13
Question
Please see the WB image, lot number and protocol we used for liver using anti-CYP2A7 antibody A10507. Please let me know if you require anything else.
L. Williams
Verified customer
Asked: 2013-04-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-26
