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Product Info Summary
| SKU: | A00530-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-CYP3A5 Antibody (C-term)
View all Cytochrome P450 3A5 Antibodies
SKU/Catalog Number
A00530-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CYP3A5 Antibody (C-term) (Catalog # A00530-1). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CYP3A5 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00530-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00530-1 is reactive to CYP3A5 in Human
Reconstitution
Calculated molecular weight
57109 Da
Background of Cytochrome P450 3A5
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00530-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of anti-CYP3A5 Antibody (C-term) (Cat. #A00530-1) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.
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Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term) (Cat. #A00530-1). A00530-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For CYP3A5 (Source: Uniprot.org, NCBI)
Gene Name
CYP3A5
Full Name
Cytochrome P450 3A5
Weight
57109 Da
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 3A5, CYPIIIA5, Cytochrome P450 HLp2, Cytochrome P450-PCN3, CYP3A5 CYP3A5 CP35, CYPIIIA5, P450PCN3, PCN3 cytochrome P450 family 3 subfamily A member 5 cytochrome P450 3A5|aryl hydrocarbon hydroxylase|cytochrome P450 HLp2|cytochrome P450, family 3, subfamily A, polypeptide 5|cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5|cytochrome P450-PCN3|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP3A5 , check out the CYP3A5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP3A5 : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP3A5 Antibody (C-term) (A00530-1)
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