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Product Info Summary
| SKU: | A00530-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-CYP3A5 Antibody (C-term)
SKU/Catalog Number
A00530-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-CYP3A5 Antibody (C-term) (Catalog # A00530-1). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CYP3A5 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00530-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CYP3A5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human CYP3A5.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00530-1 is reactive to CYP3A5 in Human
Calculated molecular weight
57.1 kDa
Background of CYP3A5
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00530-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of CYP3A5 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of anti-CYP3A5 Antibody (C-term) (Cat. #A00530-1) in 293 cell line lysates (35ug/lane). CYP3A5 (arrow) was detected using the purified Pab.
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Western blot analysis of lysate from SK-BR-3 cell line, using CYP3A5 Antibody (C-term) (Cat. #A00530-1). A00530-1 was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CYP3A5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-CYP3A5 Antibody (C-term) (A00530-1)
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