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CYP4F2 antibody (A01737-2) performs well in WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of CYP4F2 using anti-CYP4F2 antibody (A01737-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antigen affinity purified polyclonal antibody (A01737-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP4F2 at approximately 50 kDa. The expected band size for CYP4F2 is at 60 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP4F2 antigen affinity purified polyclonal antibody (A01737-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP4F2 at approximately 50 kDa. The expected band size for CYP4F2 is at 60 kDa.
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Anti-CYP4F2 Antibody Picoband®
CYP4F2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | WB |
Tested Applications
CYP4F2 antibody was positively detected by WB in:
human A549 whole cell, human HepG2 whole cell, human K562 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-CYP4F2 Antibody Picoband®
View all CYP4F2 Antibodies
View all CYP4F2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CYP4F2.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
59.9 kDa
Background of CYP4F2
Cytochrome P450 4F2 is a protein that in humans is encoded by the CYP4F2 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away.
Cite This Product
Anti-CYP4F2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01737-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
74%
Moderate Homology
Mouse
68%
Low homology
Rat
63%
Low homology
Rabbit
63%
Low homology
Cow
63%
Low homology
Sheep
63%
Low homology
Zebrafish
32%
Low homology
Drosophila
32%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP4F2 antibody
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Product Promise
We promise Anti-CYP4F2 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01737-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026