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CYP7A1 antibody (RP1079) performs well in Flow Cytometry, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of SiHa cells using anti-CYP7A1 antibody (RP1079).
Overlay histogram showing SiHa cells stained with RP1079 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP7A1 Antibody (RP1079, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing SiHa cells stained with RP1079 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP7A1 Antibody (RP1079, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-CYP7A1 Antibody Picoband®
CYP7A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | Flow Cytometry, WB |
Tested Applications
CYP7A1 antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, rat RH35 whole clel
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYP7A1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Product Information
Product Name
Anti-CYP7A1 Antibody Picoband®
View all CYP7A1 Antibodies
View all CYP7A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
57.7 kDa
Background of CYP7A1
CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA -HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.
Cite This Product
Anti-CYP7A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1079)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
96%
High Homology
Dog
88%
Moderate Homology
Cow
88%
Moderate Homology
Sheep
87%
Moderate Homology
Rabbit
85%
Moderate Homology
Mouse
85%
Moderate Homology
Rat
83%
Moderate Homology
Zebrafish
65%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP7A1 antibody
Flow Cytometry (FC) protocol for CYP7A1 antibody
Publications
Application:
Species:
Jiangzhi ruanmai recipe alleviates atherosclerosis in ApoE−/− mice by regulating cholesterol metabolism involved in gut microbiota remodeling
Published: 2026-05-05
Journal: Frontiers in Cardiovascular Medicine
Application: WB
Impact Factor: 3.00
Species: Mouse
PMID: 42291552
Protective effects and mechanism of Sangyu granule on acetaminophen-induced liver injury in mice
Published: 2024-05-01
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 38701935
QiShenYiQi pill inhibits atherosclerosis by promoting TTC39B-LXR mediated reverse cholesterol transport in liver
Published: 2023-11-05
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 7.90
Species: Rat,Mouse
PMID: 37951148
Zhuyu Pill Alleviates Nonalcoholic Fatty Liver Disease by Regulating Bile Acid Metabolism through the Gut–Liver Axis
Published: 2023-08-02
Journal: ACS Omega
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 37599938
Quercetin 7-rhamnoside protects against alpha-naphthylisothiocyanate (ANIT)-induced in cholestatic hepatitis rats by improving biliary excretion and inhibiting inflammatory responses
Published: 2023-01-09
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.60
Species: Rat
PMID: 36699093
Tissue-specific mechanisms of bile acid homeostasis and activation of FXR-FGF19 signaling in preterm and term neonatal pigs
Published: 2021-12-30
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
Application: WB
Impact Factor: 4.05
Species: Pig
PMID: 34851728
Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD
Published: 2019-04-04
Journal: JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Application: WB
Impact Factor: 4.49
Species: Mouse
PMID: 31055233
Fucoidan and galactooligosaccharides ameliorate high-fat diet–induced dyslipidemia in rats by modulating the gut microbiota and bile acid metabolism
Published: 2019-03-11
Journal: NUTRITION
Application: WB
Impact Factor: 3.59
Species: Rat
PMID: 31029922
Review
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Submit A Review1 Review For Anti-CYP7A1 Antibody Picoband®
Anti-CYP7A1 Antibody (RP1079) successfully captured CYP7A1 expression reduction in liver samples from ANIT-induced cholestatic rats via WB, with only faint off-target bands visible.
| SKU | RP1079 |
|---|---|
| Application | Western Blot |
| Sample | Liver tissue from an ANIT-induced rat model |
| Sample Processing Description | Cholestatic liver injury was induced in SD rats via intragastric administration of ANIT. Liver tissues were harvested 48 hours later, and proteins were extracted using RIPA lysis buffer. Following BCA quantification, the samples were mixed with loading buffer and boiled for denaturation. |
| Other Reagents | Blocking buffer, RIPA lysis buffer |
| Primary Antibody | COL6A1 Antibody Picoband® |
| Primary Incubation | |
| Secondary Antibody | HRP-conjugated anti-rabbit IgG secondary antibody |
| Secondary Incubation | 1:50000 |
| Detection | ECL, Imaging system: Contact-Based Non-Destructive Signal Imaging |
| Results Summary | The image shows the following lanes: Lane 1 represents the liver of control-group rats, displaying a clear and uniform CYP7A1 band; Lane 2 represents the ANIT-treated group; and Lane 3 represents the drug-treated group, where the CYP7A1 band is markedly reduced. This demonstrates that the antibody effectively detects changes in CYP7A1 expression. |
Yin Zhou, The Fifth Medical Center of Chinese PLA General Hospital
Verified customer
Submitted 2026-09-16
Product Promise
We promise Anti-CYP7A1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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RP1079
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 21, 2026