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Product Info Summary
| SKU: | A05518-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CYP8B1 Antibody
SKU/Catalog Number
A05518-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CYP8B1 Antibody catalog # A05518-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP8B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05518-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 8B1. AA range:371-420
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05518-1 is reactive to CYP8B1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
58.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05518-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using CYP8B1 Polyclonal Antibody
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Western blot analysis of Cytochrome P450 8B1 Antibody. The lane on the right is blocked with the Cytochrome P450 8B1 peptide.
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Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 8B1 antibody.
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Immunohistochemistryt analysis of paraffin-embedded human liver, using Cytochrome P450 8B1 Antibody. The lane on the right is blocked with the Cytochrome P450 8B1 peptide.
Specific Publications For Anti-CYP8B1 Antibody (A05518-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CYP8B1 Antibody
Question
Will anti-CYP8B1 antibody A05518-1 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to the expression profile of liver, CYP8B1 is highly expressed in liver. So, it is likely that anti-CYP8B1 antibody A05518-1 will work for WB with liver.
Boster Scientific Support
Answered: 2019-05-21
Question
Is this A05518-1 anti-CYP8B1 antibody reactive to the isotypes of CYP8B1?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
The immunogen of A05518-1 anti-CYP8B1 antibody is Synthesized peptide derived from the Internal region of human CYP8B1. at AA rangle: 340-420. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-14
Question
Is there a BSA free version of anti-CYP8B1 antibody A05518-1 available?
Verified Customer
Verified customer
Asked: 2017-11-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CYP8B1 antibody A05518-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-06
Question
We are currently using anti-CYP8B1 antibody A05518-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
W. Miller
Verified customer
Asked: 2017-10-10
Answer
The anti-CYP8B1 antibody (A05518-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-10
Question
Will anti-CYP8B1 antibody A05518-1 work on bovine WB with liver?
J. Jackson
Verified customer
Asked: 2016-12-12
Answer
Our lab technicians have not tested anti-CYP8B1 antibody A05518-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CYP8B1 antibody A05518-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-12-12
Question
See below the WB image, lot number and protocol we used for liver using anti-CYP8B1 antibody A05518-1. Please let me know if you require anything else.
R. Carter
Verified customer
Asked: 2014-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-31
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CYP8B1 antibody A05518-1. Let me know if you need anything else.
C. Evans
Verified customer
Asked: 2014-07-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-03
