Anti-CYP8B1 Antibody

CYP8B1 antibody

Boster Bio Anti-CYP8B1 Antibody catalog # A05518-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05518-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-CYP8B1 Antibody

View all CYP8B1 Antibodies

SKU/Catalog Number

A05518-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CYP8B1 Antibody catalog # A05518-1. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CYP8B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05518-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human CYP8B1. at AA rangle: 340-420

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05518-1 is reactive to CYP8B1 in Human, Mouse

Applications

A05518-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

Gene/Protein Information For CYP8B1 (Source: Uniprot.org, NCBI)

Uniprot ID

Q9UNU6

Gene ID

1582

Gene Name

CYP8B1

Full Name

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Weight

58.068kDa

Superfamily

cytochrome P450 family

Alternative Names

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase CYP8B1 CP8B, CYP12 cytochrome P450 family 8 subfamily B member 1 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase|7 alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase|CYPVIIIB1|cytochrome P450 8B1|cytochrome P450, family 8, subfamily B, polypeptide 1|cytochrome P450, subfamily VIIIB (sterol 12-alpha-hydroxylase), polypeptide 1|sterol 12-alpha-hydroxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP8B1, check out the CYP8B1 Infographic

CYP8B1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP8B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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7 Customer Q&As for Anti-CYP8B1 Antibody

Question

Will anti-CYP8B1 antibody A05518-1 work for WB with liver?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

According to the expression profile of liver, CYP8B1 is highly expressed in liver. So, it is likely that anti-CYP8B1 antibody A05518-1 will work for WB with liver.

Boster Scientific Support

Answered: 2019-05-21

Question

Is this A05518-1 anti-CYP8B1 antibody reactive to the isotypes of CYP8B1?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

The immunogen of A05518-1 anti-CYP8B1 antibody is Synthesized peptide derived from the Internal region of human CYP8B1. at AA rangle: 340-420. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-14

Question

Is there a BSA free version of anti-CYP8B1 antibody A05518-1 available?

Verified Customer

Verified customer

Asked: 2017-11-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CYP8B1 antibody A05518-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-06

Question

We are currently using anti-CYP8B1 antibody A05518-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?

W. Miller

Verified customer

Asked: 2017-10-10

Answer

The anti-CYP8B1 antibody (A05518-1) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-10

Question

Will anti-CYP8B1 antibody A05518-1 work on bovine WB with liver?

J. Jackson

Verified customer

Asked: 2016-12-12

Answer

Our lab technicians have not tested anti-CYP8B1 antibody A05518-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CYP8B1 antibody A05518-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-12-12

Question

See below the WB image, lot number and protocol we used for liver using anti-CYP8B1 antibody A05518-1. Please let me know if you require anything else.

R. Carter

Verified customer

Asked: 2014-10-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-31

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CYP8B1 antibody A05518-1. Let me know if you need anything else.

C. Evans

Verified customer

Asked: 2014-07-03

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-07-03

Size

Total: $399

SKU:A05518-1

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A05518-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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