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Product Info Summary
| SKU: | A02794 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cystatin B (N84) CSTB Antibody
View all Cystatin B/Stefin B Antibodies
SKU/Catalog Number
A02794
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cystatin B (N84) CSTB Antibody catalog # A02794. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cystatin B (N84) CSTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02794)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ROM-K around the phosphorylation site of S44.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02794 is reactive to CSTB in Human, Mouse, Rat
Applications
A02794 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
11.14kDa
Background of Cystatin B/Stefin B
The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARFof CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARFof CDKN2A By similarity. Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Hiroyasu Inada, J. Cell Biol., Oct 2001; 155: 415.
Kenneth M. Izumi, Mol. Cell. Biol., Aug 1999; 19: 5759 - 5767.
Susumu Rokudai, Mol. Cell. Biol., Dec 2002; 22: 8695 - 8708.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of CSTB using Anti-Cystatin B (N84) CSTB Antibody (A02794).
Immunohistochemistry (IHC) analyzes of Cystatin B (N84) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Cystatin B (N84) CSTB Antibody A02794
Western blot (WB) analysis of Cystatin B (N84) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate
Lane2:Raw264.7 cell lysate
Lane3:PC12 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CSTB (Source: Uniprot.org, NCBI)
Gene Name
CSTB
Full Name
Cystatin-B
Weight
11.14kDa
Superfamily
cystatin family
Alternative Names
CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B CSTB CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD cystatin B cystatin-B|cystatin B (stefin B)|epididymis secretory sperm binding protein|liver thiol proteinase inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSTB, check out the CSTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cystatin B (N84) CSTB Antibody (A02794)
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No publications found for A02794
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