Product Info Summary
SKU: | PA2106 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Stefin B/CSTB Antibody
View all Cystatin B/Stefin B Antibodies
SKU/Catalog Number
PA2106
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Stefin B/CSTB Antibody catalog # PA2106. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Stefin B/CSTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2106)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Stefin B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2106 is reactive to CSTB in Human
Applications
PA2106 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
14 kDa
Calculated molecular weight
11.14kDa
Background of Cystatin B/Stefin B
Cystatin B (CSTB),also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is though to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Stefin B antibody, PA2106, Western blotting
Lane 1: A549 Cell Lysate
Lane 2: A431 Cell Lysate
Lane 3: COLO320 Cell Lysate
Lane 4: HELA Cell Lysate
Click image to see more details
Anti-Stefin B antibody, PA2106, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CSTB (Source: Uniprot.org, NCBI)
Gene Name
CSTB
Full Name
Cystatin-B
Weight
11.14kDa
Superfamily
cystatin family
Alternative Names
CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B CSTB CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD cystatin B cystatin-B|cystatin B (stefin B)|epididymis secretory sperm binding protein|liver thiol proteinase inhibitor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSTB, check out the CSTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Stefin B/CSTB Antibody (PA2106)
Hello CJ!
No publications found for PA2106
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Stefin B/CSTB Antibody
Question
Is this PA2106 anti-Stefin B/CSTB antibody reactive to the isotypes of CSTB?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The immunogen of PA2106 anti-Stefin B/CSTB antibody is A synthetic peptide corresponding to a sequence in the middle region of human Stefin B(26-45aa QLEEKENKKFPVFKAVSFKS). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mouth mucosa using anti-Stefin B/CSTB antibody PA2106. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
I have attached the WB image, lot number and protocol we used for mouth mucosa using anti-Stefin B/CSTB antibody PA2106. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-01
Question
you antibody to test anti-Stefin B/CSTB antibody PA2106 on human mouth mucosa for research purposes, then I may be interested in using anti-Stefin B/CSTB antibody PA2106 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Walker
Verified customer
Asked: 2017-08-03
Answer
The products we sell, including anti-Stefin B/CSTB antibody PA2106, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-03
Question
Do you have a BSA free version of anti-Stefin B/CSTB antibody PA2106 available?
Verified Customer
Verified customer
Asked: 2017-07-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Stefin B/CSTB antibody PA2106 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-14
Question
I was wanting to use your anti-Stefin B/CSTB antibody for IHC for human mouth mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mouth mucosa identification?
D. Thomas
Verified customer
Asked: 2015-02-11
Answer
As indicated on the product datasheet, PA2106 anti-Stefin B/CSTB antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human mouth mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-11
Question
Will anti-Stefin B/CSTB antibody PA2106 work for IHC with mouth mucosa?
Z. Mitchell
Verified customer
Asked: 2014-09-30
Answer
According to the expression profile of mouth mucosa, CSTB is highly expressed in mouth mucosa. So, it is likely that anti-Stefin B/CSTB antibody PA2106 will work for IHC with mouth mucosa.
Boster Scientific Support
Answered: 2014-09-30