Anti-Cysteine protease ATG4C ATG4C Antibody

Product Name

Anti-Cysteine protease ATG4C ATG4C Antibody

View all ATG4C Antibodies

SKU/Catalog Number

A09728

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Cysteine protease ATG4C ATG4C Antibody (Catalog # A09728). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

ATG4C antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Cysteine protease ATG4C ATG4C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09728)

Host

Rabbit

Contents

ATG4C antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C. The immunogen is located within the last 50 amino acids of ATG4C.

Cross-reactivity

ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.

Reactive Species

A09728 is reactive to ATG4C in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

52.5 kDa

Background of ATG4C

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09728 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information ATG4C

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