Click image to see more details
Product Info Summary
| SKU: | M00598 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody
SKU/Catalog Number
M00598
BM5669 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody catalog # M00598. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00598)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFAI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome P450 1A2
Reactive Species
M00598 is reactive to CYP1A2 in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
58.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00598 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:500
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Cytochrome P450 1A2 expression in Caco2 cell lysate.
Specific Publications For Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody (M00598)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal Antibody
Question
Is this M00598 anti-Cytochrome P450 1A2 Monoclonal antibody reactive to the isotypes of CYP1A2?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The immunogen of M00598 anti-Cytochrome P450 1A2 Monoclonal antibody is A synthesized peptide derived from human Cytochrome P450 1A2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-28
Question
Is a blocking peptide available for product anti-Cytochrome P450 1A2 Monoclonal antibody (M00598)?
N. Wu
Verified customer
Asked: 2020-04-22
Answer
We do provide the blocking peptide for product anti-Cytochrome P450 1A2 Monoclonal antibody (M00598). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-22
Question
Does anti-Cytochrome P450 1A2 Monoclonal antibody M00598 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
Our lab technicians have not tested anti-Cytochrome P450 1A2 Monoclonal antibody M00598 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Cytochrome P450 1A2 Monoclonal antibody M00598 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-06
Question
I see that the anti-Cytochrome P450 1A2 Monoclonal antibody M00598 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-12
Question
I was wanting to use your anti-Cytochrome P450 1A2 Monoclonal antibody for ICC for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, M00598 anti-Cytochrome P450 1A2 Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-20
Question
I have a question about product M00598, anti-Cytochrome P450 1A2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00598 anti-Cytochrome P450 1A2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-01
Question
We are interested in to test anti-Cytochrome P450 1A2 Monoclonal antibody M00598 on human liver for research purposes, then I may be interested in using anti-Cytochrome P450 1A2 Monoclonal antibody M00598 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
The products we sell, including anti-Cytochrome P450 1A2 Monoclonal antibody M00598, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-01
