Product Info Summary
SKU: | A14222 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Cytochrome P450 20A1 CYP20A1 Antibody
SKU/Catalog Number
A14222
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 20A1 CYP20A1 Antibody (Catalog # A14222). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 20A1 CYP20A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14222)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Cytochrome P450 20A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14222 is reactive to CYP20A1 in Human
Applications
A14222 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.432kDa
Background of CYP20A1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen.
Clark H.F., Genome Res. 13:2265-2270(2003).
Hillier L.W., Nature 434:724-731(2005).
Colinge J., Submitted (OCT-2008) to UniProtKB.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 20A1 antibody A14222.
Protein Target Info & Infographic
Gene/Protein Information For CYP20A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP20A1
Full Name
Cytochrome P450 20A1
Weight
52.432kDa
Superfamily
cytochrome P450 family
Alternative Names
CYP-M; cytochrome P450 20A1; cytochrome P450 monooxygenase; cytochrome P450, family 20, subfamily A, polypeptide 1; EC 1.14; MGC22229 CYP20A1 CYP-M cytochrome P450 family 20 subfamily A member 1 cytochrome P450 20A1|cytochrome P450 monooxygenase|cytochrome P450, family 20, subfamily A, polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP20A1, check out the CYP20A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP20A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 20A1 CYP20A1 Antibody (A14222)
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No publications found for A14222
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Cytochrome P450 20A1 CYP20A1 Antibody
Question
Is this A14222 anti-Cytochrome P450 20A1 antibody reactive to the isotypes of CYP20A1?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The immunogen of A14222 anti-Cytochrome P450 20A1 antibody is Synthesized peptide derived from internal of human Cytochrome P450 20A1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-21
Question
I would like to test anti-Cytochrome P450 20A1 antibody A14222 on human blood lung for research purposes, then I may be interested in using anti-Cytochrome P450 20A1 antibody A14222 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
The products we sell, including anti-Cytochrome P450 20A1 antibody A14222, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-11
Question
We are currently using anti-Cytochrome P450 20A1 antibody A14222 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Z. Baker
Verified customer
Asked: 2013-02-13
Answer
The anti-Cytochrome P450 20A1 antibody (A14222) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-13