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Product Info Summary
| SKU: | A14660 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-HHATL Antibody
SKU/Catalog Number
A14660
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-HHATL Antibody (Catalog # A14660). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
HHATL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-HHATL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14660)
Host
Rabbit
Contents
HHATL Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HHATL antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HHATL. The immunogen is located within the first 50 amino acids of HHATL.
Reactive Species
A14660 is reactive to HHATL in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.7 kDa
Background of HHATL
The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14660 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
HHATL antibody can be used for detection of HHATL by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of HHATL in 3T3 cell lysate with HHATL antibody at 1 μg/mL.
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Immunohistochemistry of HHATL in human skin tissue with HHATL antibody at 2.5 μg/mL.
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Immunofluorescence of HHATL in human skin tissue with HHATL antibody at 20 μg/mL.
Specific Publications For Anti-HHATL Antibody (A14660)
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