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Product Info Summary
| SKU: | M00672-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1)
SKU/Catalog Number
M00672-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) catalog # M00672-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00672-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
2C7G1
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00672-2 is reactive to CYP2E1 in Human, Mouse, Rat
Observed Molecular Weight
56 kDa
Calculated molecular weight
56.8 kDa
Background of CYP2E1
Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00672-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Positive Control
WB: human HCCP tissue, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of Cytochrome P450 2E1/CYP2E1 using anti-Cytochrome P450 2E1/CYP2E1 antibody (M00672-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome P450 2E1/CYP2E1 antigen affinity purified monoclonal antibody (Catalog # M00672-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytochrome P450 2E1/CYP2E1 at approximately 56 kDa. The expected band size for Cytochrome P450 2E1/CYP2E1 is at 56 kDa.
Specific Publications For Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) (M00672-2)
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